Artificial intelligence in triage of COVID-19 patients.

In 2019, COVID-19 began one of the greatest public health challenges in history, reaching pandemic status the following year. Systems capable of predicting individuals at higher risk of progressing to severe forms of the disease could optimize the allocation and direction of resources. In this work, we evaluated the performance of different Machine Learning algorithms when predicting clinical outcomes of patients hospitalized with COVID-19, using clinical data from hospital admission alone.

Initial panel of immunocytochemical markers for identification of primary carcinoma site for effusions and peritoneal washings from women.

Introduction: In women, most malignant effusions are from breast and ovary primary carcinomas that have metastasized to body cavity fluids (pleural, peritoneal and pericardial). When carcinoma is diagnosed in effusions, it is not possible to identify its site of origin solely by cytology (morphology); therefore, immunocytochemistry is used as a complementary method. There are no immunocytochemical markers with 100% sensitivity and specificity for identifying carcinoma primary site.

Cervical Cytology of Samples with , , , , , and Detected by Multiplex PCR.

Introduction: Despite increasing application of molecular diagnostic methods for the detection of sexually transmitted infections, the cytological findings in pap smears of patients with pathogens that can be identified only by PCR are not yet well described. The aim of this study was to describe the most common cytological features in cervical pap smears of patients with , , , , , , and detected by multiplex PCR.

Methods: Cervical samples for conventional and liquid-based cytology and for multiplex PCR were collected from women ranging from 23 to 54 years old, who underwent routine screening at a gynecological Unit.

Anti-EpCAM antibodies for detection of metastatic carcinoma in effusions and peritoneal wash.

Epithelial cell adhesion molecule (EpCAM) has been used as diagnostic/prognostic marker and therapeutic target. The aim of the present study was to compare immunoreactivity of antibodies against distinct epitopes in the ectodomain of EpCAM for detection of carcinoma from different primary sites and of different histological types in effusions and peritoneal wash. Two antibodies against epitopes in the EGF-like domain I (clones Moc-31 and Ber-EP4) and one antibody against the epitope in the cysteine-poor region (158210) of EpCAM were used (all commercially available).

Special Phenomena in Rorschach Protocols in Patients Diagnosed with Schizophrenia.

The Rorschach method has been proved as an effective tool for personality dynamics assessment, as well as for identify psychopathological patterns, especially with regard to the dissociation of consciousness, which makes it relevant in the context of schizophrenia. The aim of this study was to contribute to the evaluation of the so-called special phenomena in Rorschach protocols in patients diagnosed with schizophrenia. The sample consisted of ten patients previously diagnosed with ICD-10 F20 who were hospitalised in the Federal District, Brazil, in the Centre for Living and Psychosocial Care (Life Mansion).

Role of voltage-gated calcium channels on striatal dopamine release induced by inorganic mercury in freely moving rats.

The possible role of voltage-sensitive calcium channels (VSCC) activation on the HgCl-induced dopamine release was investigated using selective VSCC blockers and the dopamine levels were measured by HPLC from samples obtained by in vivo brain microdialysis. Infusion of HgCl in nicardipine (10 or 100 μM) or flunaricine (10 μM) pretreated animals had no significant effect on dopamine release induced by HgCl. Pretreatment with 100 μM flunaricine, 20 μM ω-conotoxin MVIIC, or ω-conotoxin GVIA significantly decreased the HgCl-induced dopamine release over 61%, 88%, and 99%, respectively.

A panel of markers for identification of malignant and non-malignant cells in culture from effusions.

The aim of the present study was to identify cell types in primary culture from malignant and non-malignant effusions. Effusion samples were subjected to cytology and culture. Immunocytochemistry was performed in cytological slides to evaluate malignancy (positivity for malignancy markers) and in culture slides for identification of cell types in growth.

Hippocampal and cerebellar histological changes and their behavioural repercussions caused by brain ischaemic hypoxia experimentally induced by sodium nitrite.

Introduction: Brain ischaemic hypoxia can produce severe neurological damage that leads to behavioural disorders. This research analysed the hippocampal and cerebellar histological alterations caused by brain ischaemic hypoxia experimentally induced by sodium nitrite (NaNO) and possible direct repercussions of this hypoxia on behaviour.

Methodology: An experimental study was carried out by administering 60mg/kg NaNO to 10 Wistar rats at 3 months of age for 15 consecutive days.

Behavioural and cognitive effects of simvastatin dose used in stimulation of bone regeneration in rats.

Purpose: To analyze the effects of simvastatin (SVT) in the locomotion, anxiety and memory of rats, as a reflection of the administration of a minimum dose capable of stimulating bone regeneration in defects in the calvariae.

Methods: Surgical procedures were performed in 15 female Wistar rats, 2-month old, to insert the grafting material regenerator (Bone-ceramic®) and/or SVT, followed by behavioural and cognitive assessments in the 7th, 30th and 60th days post surgery.

Results: The SVT locally applied with the goal of bone regeneration in defects created in rat calvariae does not interfere with locomotion, anxiety levels and/or memories of rats, except for the first week following surgery, when an anxiolytic effect was observed, as a result of a possible central action.