LED based large field of view off-axis quantitative phase contrast microscopy by hologram multiplexing.

In this manuscript, we describe the development of a single shot, self-referencing wavefront division, multiplexing digital holographic microscope employing LED sources for large field of view quantitative phase imaging of biological samples. To address the difficulties arising while performing interferometry with low temporally coherent sources, an optical arrangement utilizing multiple Fresnel Biprisms is used for hologram multiplexing, enhancing the field of view and increasing the signal to noise ratio. Biprisms offers the ease of obtaining interference patterns by automatically matching the path length between the two off-axis beams.

Wavefront division digital holographic microscopy.

Digital holographic microscopy is the state of the art quantitative phase imaging of micro-objects including living cells. It is an ideal tool to image and quantify cell thickness profiles with nanometer thickness resolution. Digital holographic techniques usually are implemented using a two-beam setup that may be bulky and may not be field portable.

Wide field of view common-path lateral-shearing digital holographic interference microscope.

Quantitative three-dimensional (3-D) imaging of living cells provides important information about the cell morphology and its time variation. Off-axis, digital holographic interference microscopy is an ideal tool for 3-D imaging, parameter extraction, and classification of living cells. Two-beam digital holographic microscopes, which are usually employed, provide high-quality 3-D images of micro-objects, albeit with lower temporal stability.

Highly stable digital holographic microscope using Sagnac interferometer.

Interferometric microscopy has grown into a very potent tool for quantitative phase imaging of biological samples. Among the interfermetric methods, microscopy by digital holography is one of the most effective techniques, especially for studying dynamics of cells. Imaging of cell fluctuations requires digital holographic setups with high temporal stability.

Quantitative phase-contrast imaging with compact digital holographic microscope employing Lloyd's mirror.

Digital holographic microscopy (DHM) is one of the most effective techniques used for quantitative phase imaging of cells. Here we present a compact, easy to implement, portable, and very stable DHM setup employing a self-referencing Lloyd's mirror configuration. The microscope is constructed using a diode laser source and a CMOS sensor, making it cost effective.

High-resolution quantitative phase microscopic imaging in deep UV with phase retrieval.

High-resolution three-dimensional (3D) microscopic imaging requires the use of short wavelengths. Quantitative 3D imaging techniques, such as digital holographic microscopy, require interference between the object beam and a known reference background for the extraction of phase information. At shorter wavelengths, due to short coherence lengths, it may be difficult to implement a two-beam off-axis setup.

Quantitative cell imaging using single beam phase retrieval method.

Quantitative three-dimensional imaging of cells can provide important information about their morphology as well as their dynamics, which will be useful in studying their behavior under various conditions. There are several microscopic techniques to image unstained, semi-transparent specimens, by converting the phase information into intensity information. But most of the quantitative phase contrast imaging techniques is realized either by using interference of the object wavefront with a known reference beam or using phase shifting interferometry.

Shape and deformation measurements of 3D objects using volume speckle field and phase retrieval.

Shape and deformation measurement of diffusely reflecting 3D objects are very important in many application areas, including quality control, nondestructive testing, and design. When rough objects are exposed to coherent beams, the scattered light produces speckle fields. A method to measure the shape and deformation of 3D objects from the sequential intensity measurements of volume speckle field and phase retrieval based on angular-spectrum propagation technique is described here.

Measurement of parameters of simple lenses using digital holographic interferometry and a synthetic reference wave.

The use of digital holographic intrerferometry in the testing of simple thin lenses is explored. Focal length, radius of curvature, and refractive index are the lens parameters that can be determined using this method. The digital holograms using the lens under test are recorded at various positions of the test lens using off-axis geometry.

Determination of refractive indices of biconvex lenses by use of a Michelson interferometer.

Measurements of lens parameters such as focal length, radius of curvature, and refractive index are important. We describe a measurement method that utilizes a Michelson interferometer to determine parameters of thin, convex lenses. The real fringe system formed by a Michelson interferometer is used to determine the focal lengths and the radii of curvature of the lenses.

Diffusivity studies of transparent liquid solutions by use of digital holographic interferometry.

Real-time digital holography is used to study the diffusion process in transparent liquid solutions. Holograms of an object diffusively reflecting through an experimental cell containing diffusing solutions are recorded at different time instances. The recording medium is a CCD chip.

Collimation testing with optically active materials.

A novel method to test the collimation of laser beams with optically active mediums and a pair of crossed polarizers is presented. Optically active materials rotate the plane of polarization of incident plane-polarized light. A decollimated laser beam passing through such a material will experience a greater effective thickness than a collimated laser beam, resulting in greater outputs.