Publications by authors named "Vanessa R Palmer"
Article Synopsis
- Green fluorescence protein (GFP) is used to monitor protein expression in single cells, but its detection is complicated by autofluorescence in prokaryotes.
- The study utilizes capillary electrophoresis with laser-induced fluorescence to effectively separate GFP from native autofluorescent signals, enhancing detection capabilities.
- The results demonstrate the successful expression and detection of GFP in a single Deinococcus radiodurans bacterium, marking a significant advancement in fluorescence monitoring techniques.
Differential detergent fractionation was used to sequentially extract cytosolic, membrane, nuclear, and cytoskeletal fractions from AtT-20 cells. Extracted components were denatured by sodium dodecyl sulfate (SDS) and then labeled with the fluorogenic reagent 3-(2-furoyl) quinoline-1-carboxaldehyde. Both capillary sieving electrophoresis (CSE) and micellar electrokinetic capillary chromatography (MECC) were used to separate labeled components by one-dimensional (1D) electrophoresis.
View Article and Find Full Text PDF