Publications by authors named "Vanessa Castagna"
Characterization of the exopolysaccharides produced by the industrial yeast Komagataella phaffii.
J Ind Microbiol Biotechnol
January 2024
Article Synopsis
- The yeast Komagataella phaffii is favored by biotech startups for producing recombinant proteins due to its safety record and effective production processes, but recent findings reveal a significant polysaccharide accumulation during fermentation that complicates product purity and adds processing costs.
- The study focused on using K. phaffii strain YB-4290 to produce lactoferrin, uncovering that a considerable amount of carbohydrate (mainly composed of mannose) co-purifies with the protein, indicating challenges in purification methods.
- Further investigations into carbohydrate sources showed that commonly used strains produce baseline exopolysaccharides, with higher levels occurring under recombinant protein expression, which may inform strategies for improving efficiency in protein production with K. ph
Gut microbiome and breast-feeding: Implications for early immune development.
J Allergy Clin Immunol
September 2022
Establishment of the gut microbiome during early life is a complex process with lasting implications for an individual's health. Several factors influence microbial assembly; however, breast-feeding is recognized as one of the most influential drivers of gut microbiome composition during infancy, with potential implications for function. Differences in gut microbial communities between breast-fed and formula-fed infants have been consistently observed and are hypothesized to partially mediate the relationships between breast-feeding and decreased risk for numerous communicable and noncommunicable diseases in early life.
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