A Unified Atlas of T cell Glycophysiology.

Glycans are emerging as important regulators of T cell function but remain poorly characterized across the functionally distinct populations that exist . Here, we couple single-cell analysis technologies with soluble lectins and chemical probes to interrogate glycosylation patterns on major T cell populations across multiple mouse and human tissues. Our analysis focused on terminal glycan epitopes with immunomodulatory functions, including sialoglycan ligands for Siglecs.

Cover Feature: Direct Conjugation of Gallium-(III)-Corroles to Short Interfering RNA(siRNA) Providing Real-Time siRNA Imaging and Gene Silencing (ChemPlusChem 6/2024).

Invited for this month's cover is the group of Jean-Paul Desaulniers at Ontario Tech University. The cover picture shows the successful conjugation of a Ga-corrole to an siRNA to enable live cell imaging. Read the full text of the article at 10.

Direct Conjugation of Gallium-(III)-Corroles to Short Interfering RNA(siRNA) Providing Real-Time siRNA Imaging and Gene Silencing.

Discovering new modifications for oligonucleotide therapeutics is essential for expanding its application to new targets and diseases. In this project, we focus on conjugating metaled ligands to short interfering RNAs (siRNAs) to investigate robust and simple conjugation methods for adding new properties such as real-time imaging to the siRNA. Here we report the chemical synthesis of novel Ga-(III)-corroles for their direct conjugation to siRNAs.