Cold shock Y-box protein-1 proteolysis autoregulates its transcriptional activities.

Background: The Y-box protein-1 (YB-1) fulfills pleiotropic functions relating to gene transcription, mRNA processing, and translation. It remains elusive how YB-1 shuttling into the nuclear and cytoplasmic compartments is regulated and whether limited proteolysis by the 20S proteasome releases fragments with distinct function(s) and subcellular distribution(s).

Results: To address these questions, mapping of domains responsible for subcellular targeting was performed.