The distribution of lactate dehydrogenase isoenzymes in adult human Achilles tendons.

The authors have studied the LDH isoenzymes distribution in the human Achilles tendons. Connection between the age, sex, and enzyme activity were not found. In the Achilles tendon were found strong activity of isoenzymes IV and V (M-type), and controversy them moderate activity of isoenzymes I and II (H-type).

Histochemical and biochemical alterations in skeletal muscles of rats during combined chronic hypoxia and hypokinesia.

This investigation was designed to study the long term effect of combined hypokinesia and hypoxia on the skeletal muscle. In the muscles of the hypoxic and hypokinetic animals, serious degenerative changes were found and the fibre type ratio was alterated. The malate dehydrogenase activity was decreased, and both LDH and MDH isoenzymes pattern were alterated.

Recapillarization of tenotomized skeletal muscles after delayed tendon suture. I. Experimental study.

The investigation was under taken to study the long-term effect of tendon suturing on the capillarization of guinea-pig crural muscle after Achilles tenotomy. The results indicate that the capillary density has been greatly decreased by the tenotomy. The recapillarization and the time of restitution of the crural muscle are influenced by the interval between tenotomy and tendon suture.

The lactate dehydrogenase activity and isoenzyme pattern of normal and hypokinetic human tendons.

The LDH specific activity and the LDH isoenzyme pattern of eleven pairs of different normal tendons were compared with hypokinetic tendons. The LDH specific activity of hypokinetic tendons was 13--66% of the specific activity of normal tendons. The isoenzyme pattern of hypokinetic tendons altered in such a way that--with one exception--the ratio of LDH-5 and LDH-4 decreased and the ratio of LDH-1, LDH-2, and LDH-3 increased and, as a consequence, the amount of the M subunit decreased.

A critical study on the preparation and application of gelatin-resorcinol tissue adhesive crosslinked by formaldehyde.

A tissue adhesive composed of gelatin-resorcinol-formaldehyde was prepared. The factors, influencing its properties were discussed and compared with other preparation methods. Incised rat skin wounds were unified with adhesive applied between the wound edges or on the wound surface.

Myocardial lesions in hemorrhagic hypotension.

The myocardial alterations were investigated in dogs after hemorrhagic hypotension. Development of metabolic acidosis with increased lactate concentration was observed in all cases. Focal hemorrhages and hypoxic changes developed in the subendocarial region.

Experiences about the application of gelatin-resorcinol-formaldehyde tissue adhesive in experimental ruptured liver injury.

Ruptured liver injury was produced in dogs. For arresting the bleeding and for the tissue unifying GRF adhesive was used instead of the traditional wound treatment. The experiences obtained during the wound healing were positive.

Retransfusion acidosis and ventilation.

The blood pressure of dogs anaesthetized with 0.1 g/kg of chloralose was reduced to 40 mm Hg by arterial bleeding. Thirty percent of the drawn blood refluxed spontaneously, the rest was retransfused either suddenly arterially or slowly in 15 minutes by the venous route.

Investigation of the short-time autolysis of rat hearts by means of SDS. Polyacrylamide gel electrophoresis and electron microscopy.

The short-time autolysis of hearts was regarded as a model of ischaemic heart failure. Therefore, isolated rat hearts were subjected to 30--120 min autolysis in a Locke solution at 37 degrees C. Electron microscopic examinations and myofibrillar preparations were made from the autolysed heart ventricles.

[Myocardial damage in hemorrhagic hypotension].

Damage to the myocard caused by the loss of blood was studied in experiments on dogs. After long-lasting hypotonia focal lesions of the myocard of degenerative type have been found. Desorganization of the myofilaments and invagination of the disc could be seen in the area of the intercalar discs.

Myofibrillar proteins, enzyme activity and fibre types in human skeletal muscle.

The myofibrillar proteins and relative LC-3 content of myofibrils prepared from five different, left and right muscles of the human forearm were studied by electrophoresis in 10% SDS-polyacrylamide gels followed by densitometry. The specific K-activated ATPase activity of the myofibrillar myosin of the myofibrils was determined and the distribution of the fibre types was analyzed by histochemical methods. The qualitative pattern of the myofibrillar proteins was found to be identical in the different muscles; in the other parameters, however, a variation in a narrow range was observed.

Changes in the contractile material of human skeletal muscle following tendinous injury.

The most marked changes in the human muscle following tendinous injury can be observed in the contractile components. There is a widening of the Z-membrane, dispersion of the sarcomers, a break in their continuity, and detached bundles of myofibrils are found in the sarcoplasm. A change occurs also in the proportion of different fibre types of the muscles, in the muscle with tendinous injury a predominance of type II fibre becoming apparent.

[The post mortem alterations of myofibrilar proteins and adenosintriphosphatase activities of the skeletal muscles (author's transl)].

Immediately after the death of rabbits and at different times within 48 hours, we took out a part of the psoas muscle, from which we made myofibrilar preparations. The carcasses providing the muscle samples were held at two different temperatures. One group was held for 48 hours at 25 degrees C, imitating room temperature.

[Specific fibre compositon of human hand and arm muscles].

The authors examined the fiber composition of the finger, hand, and arm muscles. It is established that the type of fiber composition is not influenced by the mechanical employment of the hand- and arm muscles. The fiber composition of the finger muscles, however, is different in the two hands, in that the amount of fiber type II is significantly higher than that of the fiber type I in the dominant hand.

Retransfusion acidosis after brief haemorrhagic hypotension in the dog.

Dogs under chloralose anasthesia were bled at a rate of 50 ml/min to a total of 25 ml/kg body weight and 2 minutes later a quick reinfusion of adequate volumes of blood, dextran, or Locke's solution was done. Within 2 minutes after reinfusion, the pH of arterial blood fell by 0.074--0.

The effect of alpha-melanophor-stimulating hormone on liver regeneration and incorporation of amino acid in rats' liver protein.

Following 25 mug/day synthetic alpha-MSH administration, the liver regeneration of partially hepatectomized rats proved to be increased. The hormone treatment resulted in an enhanced alanine incorporation of the liver proteins, but this effect was uncertain on partially hepatectomized rats. Due to the hormone treatment the low liver protein content of the operated rats became normal.