Publications by authors named "Validov S"

Article Synopsis
  • Industrialization has led to significant ecological harm, prompting the exploration of sustainable green technologies that utilize microbial processes for environmental cleanup.
  • A specific strain of wheat root-associated bacteria, MGMM7, was studied for its ability to degrade pollutants like crude oil and azo dyes, demonstrating notable effectiveness in reducing these contaminants and promoting plant growth in affected soils.
  • Genomic analysis of MGMM7 revealed its unique genetic profile related to xenobiotic degradation, highlighting its potential for bioremediation in polluted environments.
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The use of metal and metal oxide nanoparticles is frequently regarded as a potential solution to the issue of bacterial antibiotic resistance. Among the proposed range of nanoparticles with antibacterial properties, copper oxide nanoparticles are of particular interest. Although the antibacterial properties of copper have been known for a considerable period of time, studies on the effects of copper oxide nanomaterials with respect to biological systems have attracted considerable attention in recent years.

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GTPase Era from Staphylococcus aureus belongs to the TRAFAC superfamily of the TrmE-Era-EngA-EngB-Septin-like GTPases class and plays a significant role in the vital activity of this pathogenic microorganism as a maturation factor of the 30S ribosome subunit. However, the functions of this protein are not fully understood, making it a promising object for further study. Here, the 2.

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  • Plant pathogens significantly affect onion crop production by reducing yield and quality, especially during long-term storage when latent infections can emerge.
  • The study focused on isolating and identifying harmful strains from yellow onion bulbs, finding that 16 strains were predominantly from the complex species responsible for disease symptoms.
  • Results indicated variations in how aggressive these strains were towards onion bulbs and seedlings, with some showing potential for environmentally friendly control methods to manage fungal growth and prevent spoilage.
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  • The YsxC protein in Staphylococcus aureus belongs to a family of GTP-binding proteins crucial for assembling the 50S ribosomal subunit, highlighting its role in the bacteria's life cycle.
  • Recent studies suggest that targeting YsxC could lead to the development of new selective antimicrobials due to its unique structural features.
  • Researchers cloned the ysxC gene, overexpressed it in E. coli, and successfully purified and crystallized the protein, achieving high-quality diffraction data for comparison with similar proteins.
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  • Anthropogenic pollution has worsened environmental issues despite efforts from the green revolution aimed at food security.
  • The research focuses on the MGMM8 strain from winter wheat, highlighting its genome annotation and potential for bioremediation and antimicrobial applications.
  • Gene analysis revealed clusters related to antibiotics, heavy metal resistance, and quorum-quenching, suggesting MGMM8 could be significant for biocontrol strategies and environmental remediation.
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Microbial biotechnology plays a crucial role in improving industrial processes, particularly in the production of compounds with diverse applications. In this study, we used bioinformatic approaches to analyze the genomic architecture of MGMM6 and identify genes involved in various metabolic pathways that have significant biotechnological potential. Genome mining revealed that MGMM6 consists of a linear chromosome of 6,932,303 bp, with a high G+C content of 73.

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Potential genotoxicity and carcinogenicity of carbon nanotubes (CNT), as well as the underlying mechanisms, remains a pressing topic. The study aimed to evaluate and compare the genotoxic effect and mechanisms of DNA damage under exposure to different types of CNT. Immortalized human cell lines of respiratory origin BEAS-2B, A549, MRC5-SV40 were exposed to three types of CNT: MWCNT Taunit-M, pristine and purified SWCNT TUBALL™ at concentrations in the range of 0.

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  • Ribosome biogenesis is a complex and energy-demanding process, where any small errors can lead to serious consequences for the cell, including death.
  • Biogenesis factors like RimP play a crucial role in this process by assisting the proper assembly of ribosomal proteins and rRNA.
  • This study details how RimP interacts with the 30S ribosomal subunit in Staphylococcus aureus, providing insights into its binding and structural dynamics through advanced techniques like cryo-EM and NMR spectroscopy.
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species have gained much attention based on their phenotypic characteristics and their genetic architecture as biological control agents and plant growth-promotor with bioremediation potential. In this study, we analyzed the whole genome of a novel strain, MGMM1, isolated from the rhizosphere of a weed plant () and assayed its phenotypic characteristics, as well as antifungal and biocontrol ability. The whole genome analysis of MGMM1 identified 4259 putative coding sequences, with an encoding density of 95.

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  • Ribosome biogenesis is a detailed process involving the maturation of ribosomal subunits, which requires the coordination of various proteins, RNAs, and enzymes.
  • The study focuses on ribosomal binding factor A (RbfA), detailing its crystal and NMR structures as well as a cryo-EM visualization of the 30S-RbfA complex.
  • The findings reveal that RbfA's role in ribosomal subunit maturation is similar in bacteria and mitochondria, suggesting potential targets for developing new antibiotics against bacterial infections.
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Competition for nutrients and niches (CNN) is known to be one of the mechanisms for biocontrol mostly exhibited by strains. Phenotypic and full genome analysis revealed PCL1760 controlling tomato foot and root rot (TFRR) solely through CNN mechanism. Although the availability of nutrients and motility are the known conditions for CNN, persistence of bacteria through dormancy by ribosomal hibernation is a key phenomenon to evade both biotic and abiotic stress.

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  • Changes in the oral microbiome occur at the onset of rheumatoid arthritis (RA), but the microbiome's state before RA in individuals with clinically suspect arthralgia (CSA) is still unclear.
  • A study involving Tatarstan women analyzed oral samples from various CSA stages and established RA patients, revealing decreased levels of specific bacterial genera in ACPA-positive individuals, especially those with early RA.
  • Findings indicate a relationship between changes in the oral microbiome and ACPA levels, suggesting a need for further research into how ACPA influences the oral microbiome in RA patients.
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  • Ribosomal silencing factor S (RsfS) is a key protein that helps shut down ribosomes and supports cell survival during starvation, and is essential for the formation of the large ribosomal subunit.* -
  • RsfS inhibits the binding of the uL14 ribosomal protein to the large subunit, preventing it from joining with the small subunit.* -
  • Recent experiments identified critical amino acid Y98 on RsfS that could be a new target for drug development aimed at treating infections.*
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The use of microorganism-based products in agricultural practices is gaining more interest as an alternative to chemical methods due to their non-toxic bactericidal and fungicidal properties. Various factors influence the efficacy of the microorganisms used as biological control agents in infield conditions as compared to laboratory conditions due to ecological and physiological aspects. Abiotic factors have been shown to trigger phase variations in bacterial microorganisms as a mechanism for adapting to hostile environments.

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  • RimP is a crucial 17.7 kDa protein necessary for the maturation and efficient processing of the 30S ribosome's 16S rRNA, being involved in early assembly stages.
  • It helps stabilize the central pseudoknot during the initial steps of 30S subunit maturation, although the exact mechanism remains unclear.
  • The study details the chemical shift assignments of RimP from Staphylococcus aureus, revealing its structure, which includes eight β-strands and three α-helices, and aims to facilitate the discovery of selective inhibitors for bacterial translation.
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  • Fusarium oxysporum strains show host specificity, important for their classification and understanding in plant pathology.
  • A specific strain (F. oxysporum f.sp. radicis-cucumerinum V03-2 g) normally infecting cucumbers has developed a new ability to also infect tomatoes while still affecting cucumbers.
  • This is the first documented case of such a change in host specificity within F. oxysporum, and the clonal derivatives can help identify the genetic factors responsible for these pathogenic changes.
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  • A widespread commensal fungus poses a significant health risk due to its increasing resistance to current antifungal medications, particularly cycloheximide (CHX).
  • The lack of structural information has hampered the understanding of this resistance and the development of new treatment options.
  • Researchers successfully determined the structure of the ribosome and its complexes with inhibitors, revealing a key change in a specific ribosomal protein that accounts for CHX resistance, paving the way for future antifungal drug innovations.
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Candida albicans (C. albicans) is a fungal pathogen that causes infections of the wet body surfaces and the blood in immunocompromised patients or individuals with imbalanced microflora. Since the cases of clinically meaningful candidosis are on the rise, efficient С.

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In this work, we, for the first time, manufactured a plasma-chemical reactor operating at a frequency of 0.11 MHz. The reactor allows for the activation of large volumes of liquids in a short time.

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Plant-protecting Bacillus sp. strains used as biocontrol agents frequently produce metabolites inhibiting phytopathogenic fungi. Recently, the search for a novel biocontrol agent with a wide spectrum of disease control drew attention to Bacillus subtilis and their related species, including Bacillus mojavensis.

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In response to stress, eubacteria reduce the level of protein synthesis and either disassemble ribosomes into the 30S and 50S subunits or turn them into translationally inactive 70S and 100S complexes. This helps the cell to solve two principal tasks: (i) to reduce the cost of protein biosynthesis under unfavorable conditions, and (ii) to preserve functional ribosomes for rapid recovery of protein synthesis until favorable conditions are restored. All known genes for ribosome silencing factors and hibernation proteins are located in the operons associated with the response to starvation as one of the stress factors, which helps the cells to coordinate the slowdown of protein synthesis with the overall stress response.

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Staphylococcus aureus is a bacterial pathogen and one of the leading causes of healthcare-acquired infections in the world. The growing antibiotic resistance of S. aureus obliges us to search for new drugs and treatments.

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Ribosome-binding factor A (RbfA) from Staphylococcus aureus is a cold adaptation protein that is required for the growth of pathogenic cells at low temperatures (10-15°C). RbfA is involved in the processing of 16S rRNA, as well as in the assembly and stabilization of the small 30S ribosomal subunit. Structural studies of the 30S-RbfA complex will help to better understand their interaction, the mechanism of such complexes, and the fundamental process such as 30S subunit assembly that determines and controls the overall level of protein biosynthesis.

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Article Synopsis
  • * Elongation Factor P (EF-P) is crucial for protein synthesis and bacterial virulence, but details about its posttranslational modifications in S. aureus are limited.
  • * This study focused on expressing EF-P in S. aureus to investigate its posttranslational modifications, successfully identifying the 5-aminopentanol modification using mass spectrometry.
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