Publications by authors named "Valery Vasil'evich Aleksandrin"
Simultaneous determination of cystine and other free aminothiols in blood plasma using capillary electrophoresis with pH-mediated stacking.
Electrophoresis
March 2024
We developed a method of sensitive capillary electrophoresis using UV detection for the determination of certain free aminothiols (reduced cysteinylglycine (rCysGly), cysteine (rCys), glutathione (rGln), and cystine (CysS) in human blood plasma. The reduced thiols were derivatized with N-ethylmaleimide. The plasma was purified from proteins via ultrafiltration.
View Article and Find Full Text PDFCoronary artery disease (CAD) and the coronary artery bypass graft (CABG) are associated with a decreased blood glutathione (bGSH) level. Since GSH metabolism is closely related to other aminothiols (homocysteine and cysteine) and glucose, the aim of this study was to reveal the associations of bGSH with glucose and plasma aminothiols in CAD patients (N = 35) before CABG and in the early postoperative period. Forty-three volunteers with no history of cardiovascular disease formed the control group.
View Article and Find Full Text PDFArticle Synopsis
- A new method using capillary electrophoresis has been developed to accurately measure reduced glutathione (GSH) and oxidized glutathione disulfide (GSSG) in whole blood.
- The process includes stable sample preparations and a fast analysis time of only 8 minutes, with quantification limits for GSSG at 1.5 µM.
- Results from healthy volunteers show average levels of GSH, GSSG, and their ratio, indicating that this approach is suitable for clinical and laboratory applications.
Objective: S-Adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) are indicators of global transmethylation and may play an important role as markers of severity of COVID-19.
Methods: The levels of plasma SAM and SAH were determined in patients admitted with COVID-19 ( = 56, mean age = 61). Lung injury was identified by computed tomography (CT) in accordance with the CT0-4 classification.