Molecular Epidemiology of Antibiotic Resistance Genes and Virulence Factors in Multidrug-Resistant Isolated from Rodents, Humans, Chicken, and Household Soils in Karatu, Northern Tanzania.

The interaction of rodents with humans and chicken in the household environment can facilitate transmission of multidrug-resistant (MDR) (), causing infections that are difficult to treat. We investigated the presence of genes encoded for carbapenem, extended spectrum beta-lactamases (ESBL), tetracycline and quinolones resistance, and virulence among 50 MDR isolated from human ( = 14), chicken ( = 12), rodent ( = 10), and soil ( = 14) samples using multiplex polymerase chain reaction (PCR). Overall, the antimicrobial resistance genes (ARGs) detected were: 23/50 (46%), 13/50 (26%), 23/50 (46%), 7/50 (14%), 12/50 (24%), 4/50 (8%), 6/50 (12%), and 3/50 (6%), while , , and were not found.

Occurrence of Multi-Drug-Resistant in Chickens, Humans, Rodents and Household Soil in Karatu, Northern Tanzania.

We investigated antibiotic resistance profiles of among 960 samples obtained from chickens (236), humans (243), rodents (101) and soil (290). was isolated from 650 (67.7%) samples.

Occurrence of Multidrug-Resistant among Humans, Rodents, Chickens, and Household Soils in Karatu, Northern Tanzania.

We conducted this study to investigate the isolation frequency and phenotypic antibiotic resistance pattern of isolated from rodents, chickens, humans, and household soils. Specimens were plated onto mannitol salt agar (Oxoid, Basingstoke, UK) and incubated aerobically at 37 °C for 24 h. Presumptive colonies of were subjected to Gram staining, as well as catalase, deoxyribonuclease (DNAse), and coagulase tests for identification.