Universal Actuator for Efficient Silencing of Genes Based on Convergent Transcription Resistant to Rho-Dependent Termination.

Dynamic control is a distinguished strategy in modern metabolic engineering, in which inducible convergent transcription is an attractive approach for conditional gene silencing. Instead of a simple strong "reverse" () promoter, a three-component actuator has been developed for constitutive genes silencing. These actuators, consisting of promoters with different strengths, the ribosomal transcription antitermination-inducing sequence -AT, and the RNase III processing site, were inserted into the 3'-UTR of three metabolic genes.