Case study on climate change effects and food security in Southeast Asia.

Agriculture, a cornerstone of human civilization, faces rising challenges from climate change, resource limitations, and stagnating yields. Precise crop production forecasts are crucial for shaping trade policies, development strategies, and humanitarian initiatives. This study introduces a comprehensive machine learning framework designed to predict crop production.

Transforming growth factor-β mimics the key proteome properties of CD133 differentiated and CD133 cancer stem cells in glioblastoma.

Glioblastoma multiforme is the most aggressive type of primary brain tumor in humans. Its invasive growth is associated with cluster of differentiation (CD)133 cancer stem cells (CSCs) and CD133 differentiated glioblastoma cells (DGCs) with aggressive phenotype, which are developed under the influence of transforming growth factor (TGF)-β. The present study aimed to compare the proteomes of CD133 CSCs and CD133 DGCs stimulated by TGF-β, as well as the expression levels of the main proteins responsible for activating the signaling pathway of receptor interactions with the extracellular matrix (ECM).

Proteins of Wnt signaling pathway in cancer stem cells of human glioblastoma.

Rationale: Glioblastoma multiforme (GBM) is the most aggressive primary glial brain tumor. The prognosis for GBM patients is not favorable, with the median survival time being 15 months. Its treatment resistance is associated with GBM cell population having cancer stem cells (CSCs).

Molecular determinants of the interaction between glioblastoma CD133 cancer stem cells and the extracellular matrix.

Glioblastoma multiforme (GBM) is the most common primary tumor of the human brain. It is characterized by invasive growth and strong resistance to treatment, and the median survival time of patients is 15 months. The invasive growth of this tumor type is associated with tumor cells with an aggressive phenotype, while its treatment resistance is attributed to cancer stem cells (CSCs).

Hematopoietic stem cells as a tool for the treatment of glioblastoma multiforme.

Glioblastoma multiforme is an aggressive malignant brain tumor with terminal consequences. A primary reason for its resistance to treatment is associated with cancer stem cells (CSCs), of which there are currently no effective ways to destroy. It remains unclear what cancer cells become a target of stem cell migration, what the role of this process is in oncogenesis and what stem cell lines should be used in developing antitumor technologies.

Molecular mechanisms of the effect of TGF-β1 on U87 human glioblastoma cells.

Glioblastoma multiforme (GBM) is the most widespread and aggressive type of primary brain tumor. The prognosis following diagnosis with GBM is poor, with a median survival time of 14 months. Tumor cell invasion, metastasis and proliferation are the major causes of mortality in patients with GBM.

To the novel paradigm of proteome-based cell therapy of tumors: through comparative proteome mapping of tumor stem cells and tissue-specific stem cells of humans.

We performed proteome mapping (PM), cataloging, and bioinformation analysis of protein lysates of human neural (CD133(+)) progenitor and stem cells (NPSCs) isolated from the olfactory sheath of a nose, multipotent mesenchymal (CD29(+), CD44(+), CD73(+), CD90(+), CD34(-)) stromal cells (MMSCs) isolated from human bone marrow, and tumor (CD133(+)) stem cells (TSCs) isolated from the human U87 glioblastoma (GB) cell line. We identified 1,664 proteins in the examined lysates of stem cells (SCs), 1,052 (63.2%) of which are identical in NPSCs and TSCs and 607 proteins (36.

Human blood plasma proteome mapping for search of potential markers of the lung squamous cell carcinoma.

Blood plasma proteomes obtained from 77 lung squamous cell carcinoma (LSCC) patients (Stages I-III) and 67 healthy controls (all males) were analyzed by using the label-free liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the search of potential cancer biomarkers. All plasma samples were depleted of 14 highly-abundant plasma proteins by immune-affinity column chromatography before LC-MS/MS. We identified and quantified 809 differential proteins with molecular weights from 6.

Identificationof potential lung cancer biomarkers by liquid chromatography tandem mass spectrometry-based proteomics analysis of secretomes of two lung cancer cell lines.

A label-free nano-liquid chromatography tandem mass spectrometry proteomics analysis on the conditioned media (CM) of two lung cancer cell lines of different histological backgrounds to identify secreted or membrane-bound proteins as novel lung cancer biomarkers was performed. Five hundred and seventy seven proteins were identified and 38% of them were classified as extracellular or membrane-bound. For the search of potential biomarkers of lung cancer a series of selection criteria were proposed.

Co-cross-linking silk matrices with silica nanostructures for robust ultrathin nanocomposites.

We report on a novel assembly approach to fabricate ultrathin robust freely standing nanocomposite membranes. The materials are composed of a pre-cross-linked silk fibroin matrix with incorporated silica nanoparticles with silsesquioxane cores (POSS) or clay nanoplatelets. These reinforced silk membranes have enhanced mechanical properties as compared to traditional silk-based nanocomposites reported previously.

Detection of lung cancer using plasma protein profiling by matrix-assisted laser desorption/ionization mass spectrometry.

There are no satisfactory plasma biomarkers which are available for the early detection and monitoring of lung cancer, one of the most frequent cancers worldwide. The aim of this study is to explore the application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS) to plasma proteomic patterns to distinguish lung cancer patients from healthy individuals. The EDTA plasma samples have been pre-fractionated using magnetic bead kits functionalized with weak cation exchange coatings.

Perforated, freely suspended layer-by-layer nanoscale membranes.

Ultrathin, perforated, and freely suspended membranes with uniform nanopores in the range of tens of nanometers have been fabricated using a fast, simple method of spin-assisted layer-by-layer assembly on hydrophobic substrates. Membranes with thicknesses down to 20 nm were robust enough to be released from the sacrificial substrates, transferred onto various surfaces, and suspended over microscopic openings. The nanopore size can be controlled by tuning the number of polyelectrolyte bilayers, spinning speed, and a proper selection of hydrophobic substrates.