DNA damage detection by Comet Assay on Daphnia magna: Application in freshwater biomonitoring.

Monitoring of water genotoxicity still remains underexploited in risk assessment. The present study aimed at standardizing and evaluating the sensitivity and applicability of the Comet Assay adapted for Daphnia magna in genotoxicological investigations in freshwater environments. Two sampling campaigns (2014-2015) were performed in the watercourses of a pilot basin located in the Parma district (Italy).

Early and late maternal effects on hatching phenology of Heterocypris incongruens (Crustacea: Ostracoda).

In ephemeral ponds, the hatching asynchrony of resting eggs may be adaptive and the result of a maternal bet-hedging strategy. A mother can influence the progeny phenology through conditions experienced during life cycle even in early development stages. We investigated the consequences of a hatching delay for offspring and compared early and late maternal effects in a clonal lineage of Heterocypris incongruens.

Comet Assay on Daphnia magna in eco-genotoxicity testing.

Detection of potentially hazardous compounds in water bodies is a priority in environmental risk assessment. For the evaluation and monitoring of water quality, a series of methodologies may be applied. Among them, the worldwide used toxicity tests with organisms of the genus Daphnia is one of the most powerful.