Publications by authors named "Valerie S Gilles"
Methanobactin (mb) is the first characterized example of a chalkophore, a class of copper-binding chromopeptides similar to iron-binding siderophores. Structural, redox, themodynamic, and spectral studies on chalkophores have focused almost exclusively on the mb from Methylosinus trichosporium OB3b (mb-OB3b). The structural characterization of a second mb from Methylocystis strain SB2 (mb-SB2) provides a means to examine the core structural features and metal binding properties of this group of chromopeptides.
View Article and Find Full Text PDFChalkophores are low molecular mass modified peptides involved in copper acquisition in methane-oxidizing bacteria (MOB). A screening method for the detection of this copper-binding molecule is presented in Chapter 16. Here we describe methods to (1) maximize expression and secretion of chalkophores, (2) concentrate chalkophores from the spent media of MOB, and (3) purify chalkophores.
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- * The study examines liver mitochondria in rats lacking the Atp7b gene, finding that copper accumulation leads to structural changes in mitochondria, such as enlarged cristae and widened intermembrane spaces, before causing functional impairments.
- * Copper exposure also resulted in specific modifications of mitochondrial proteins in a controlled environment, and treatment with copper-chelating agents helped restore mitochondrial integrity, indicating mitochondria play a critical role in the effects of copper in WD.