Hemostatically Active Proteinase Produced by : Key Specific Properties and Effect on Target Proteins.

The effect of proteinase on the proteins of the human hemostasis system, fibrin, fibrinogen, plasminogen, protein C, and factor X, was studied. These proteins are key targets for proteolytic enzymes in therapy and diagnosis of thromboembolic complications. It was shown that proteinase efficiently cleaves fibrin and fibrinogen, but does not act precisely, since it cuts all three subunits of these proteins.

Keratinolytic Properties of Promising for Biodegradation.

The high demand for keratinolytic enzymes and the modest presentation of fungal keratinase diversity studies in scientific sources cause a significant interest in identifying new fungal strains of keratinase producers, isolating new enzymes and studying their properties. Four out of the 32 cultures showed a promising target activity on protein-containing agar plates- A6, VKPM F-1593, 247, and 1779. The highest values of keratinolytic activity were demonstrated by extracellular proteins synthesized by VKPM F-1593 cultivated under submerged conditions on a medium containing milled chicken feathers.

Action of Extracellular Proteases of and Micromycetes on Plasma Hemostasis Proteins.

In this study, we investigated the properties of proteolytic enzymes of two species of , 1 (with a high degree of pathogenicity) and L-1 (a conditional pathogen), and their effects on various components of the hemostasis system (in vitro) in the case of their penetration into the bloodstream. We showed that micromycete proteases were highly active in cleaving both globular (albuminolysis) and fibrillar (fibrin) proteins, and, to varying degrees, they could coagulate the plasma of humans and animals (due to proteolysis of factors of the blood coagulation cascade) but were not able to coagulate fibrinogen. The proteases of both fully hydrolyzed thrombi in 120-180 min.

Vermiculite as a new carrier for extracellular protease production by spp. under solid-state fermentation.

A new method has been developed to increase the productivity of aspergilli - producers of extracellular proteinases based on their cultivation on vermiculite under solid-state fermentation conditions. The productivity of the mycelium L-1 and 1 was 3-18 times higher not only in comparison with submerged cultivation, but also in comparison with growth on other carriers studied under solid-state fermentation conditions. Vermiculite can be considered as a new promising carrier for solid-state fermentation of micromycetes.

Combined microbiological approach to screening of producers of proteases with hemostasis system proteins activity among micromycetes.

A scheme for screening of micromycetes - producers of proteases with the activity of hemostasis system proteins, based on their enzymatic indices determination and the activity towards chromogenic peptide substrates for proteins of the hemostasis system was developed. Depending on the ability of proteases producers to cleave such substrates, an enzymatic reaction in conditions containing human plasma is suggested, which makes it possible to identify the potentiality of the target plasma hemostasis proenzymes activation.