Seed germination and vegetative and propagation of subsp. (), a critically endangered endemic taxon of the Sicilian flora.

subsp. is a critically endangered endemic taxa of the Sicilian flora. It is a relict of the Tertiary period surviving on the cliffs of Monte Gallo (NW-Sicily).

Micropropagation of Endemic Endangered Taxa of the Italian Flora: subsp. (Asteraceae), as a Case Study.

The conservation of endangered, rare, and endemic plant species is based on in situ and ex situ conservation strategies. When in situ conservation alone is not sufficient to guarantee the survival of the species, ex situ techniques are adopted in support. This study aimed to develop an efficient micropropagation protocol for by evaluating the effect of different plant growth regulators on leaf explants.

In Vitro Rooting of L. as Affected by Genotype and by the Proliferation Method Adopted During the Multiplication Phase.

The in vitro rooting of three caper ( L.) selected biotypes, grown in a commercial orchard on the Sicilian island of Salina (38°33'49" N), was performed using-as base material for rooting experiments-shoot explants proceeding from two different in vitro culture systems: solid medium and liquid culture in a PlantForm bioreactor (TIS). The regenerated shoots of each accession were submitted to different auxin treatments (NAA, IBA, IAA - 1 or 2 mg L; NAA+IBA 0.

In Vitro Regeneration of L. by Using a Temporary Immersion System.

Three caper ( L.) biotypes grown on the Sicilian island of Salina (38°33'49″ N) were micropropagated to evaluate two different in vitro culture systems: one using the traditional solid medium, and the other based on liquid culture in a PlantForm bioreactor. PlantForm is a temporary immersion system (TIS), a new propagation method in which the shoots undergo temporary immersion in a liquid medium in order to avoid the accumulation of gas through forced ventilation.

Early embryo achievement through isolated microspore culture in Citrus clementina Hort. ex Tan., cvs. 'Monreal Rosso' and 'Nules'.

Microspore embryogenesis is a method of achieving complete homozygosity from plants. It is particularly useful for woody species, like Citrus, characterized by long juvenility, a high degree of heterozygosity and often self-incompatibility. Anther culture is currently the method of choice for microspore embryogenesis in many crops.