Integrated Profiling of Extracellular Vesicle microRNA Impact on Trabecular Meshwork mRNA Expression: Insights From Microarray Analysis.

Purpose: Extracellular vesicles (EVs) secreted by non-pigmented ciliary epithelial (NPCE) cells under oxidative stress may contribute to primary open-angle glaucoma (POAG) pathogenesis by altering gene expression in human trabecular meshwork (HTM) cells. This study investigated the impact of microRNAs (miRNAs) carried by NPCE-derived EVs on HTM cell gene expression under oxidative stress conditions.

Methods: NPCE cells were exposed to oxidative stress, and EVs were isolated from control and stressed cells.

Profiling the miRNA from Exosomes of Non-Pigmented Ciliary Epithelium-Derived Identifies Key Gene Targets Relevant to Primary Open-Angle Glaucoma.

Oxidative stress (OS) on tissues is a major pathological insult leading to elevated intraocular pressure (IOP) and primary open-angle glaucoma (POAG). Aqueous humor (AH) produced by the non-pigmentary ciliary epithelium (NPCE) drains out via the trabecular meshwork (TM) outflow pathway in the anterior chamber. The exosomes are major constituents of AH, and exosomes can modulate the signaling events, as well as the responses of their target TM tissue.