Developing a Temperature-Inducible Transcriptional Rheostat in Neurospora crassa.

Heat shock protein (HSP)-encoding genes (), part of the highly conserved heat shock response (HSR), are known to be induced by thermal stress in several organisms. In Neurospora crassa, three genes, , , and , have been characterized; however, the role of defined elements in their responses to discrete changes in temperature remains largely unexplored. To fill this gap, while also aiming to obtain a reliable fungal heat shock-inducible system, we analyzed different sections of each promoter by assessing the expression of real-time transcriptional reporters.