Nano- and microsized zeolites as a perspective material for potentiometric biosensors creation.

A number of potentiometric biosensors based on coimmobilization of enzymes with different types of zeolite on pH-ion-sensitive field-effect transistor (ISFET) have been developed. Their working characteristics have been determined and compared. It was shown that clinoptilolite and zeolite Beta polymorph A (BEA) are more promising for creating biosensors than zeolite A.

Analysis of the potato glycoalkaloids by using of enzyme biosensor based on pH-ISFETs.

The applicability of an enzyme biosensor based on pH-ISFETs for direct determination of total glycoalkaloids content in real potato samples, without any pre-treatment, is shown. The results of determination of the total glycoalkaloids concentrations in potato samples from different experimental varieties obtained by the biosensor are well correlated with the analogous data obtained by the HPLC method with standard complex sample pre-treatment procedure. The detection of total glycoalkaloids content by biosensors is reproducible, the relative standard deviation was around 3%.

Biosensors based on enzyme field-effect transistors for determination of some substrates and inhibitors.

This paper is a review of the authors' publications concerning the development of biosensors based on enzyme field-effect transistors (ENFETs) for direct substrates or inhibitors analysis. Such biosensors were designed by using immobilised enzymes and ion-selective field-effect transistors (ISFETs). Highly specific, sensitive, simple, fast and cheap determination of different substances renders them as promising tools in medicine, biotechnology, environmental control, agriculture and the food industry.

Development and optimisation of biosensors based on pH-sensitive field effect transistors and cholinesterases for sensitive detection of solanaceous glycoalkaloids.

Highly sensitive biosensors based on pH-sensitive field effect transistors and cholinesterases for detection of solanaceous glycoalkaloids have been developed, characterised and optimised. The main analytical characteristics of the biosensors developed have been studied under different conditions and an optimal experimental protocol for glycoalkaloids determination in model solution has been proposed. Using such a biosensor and an enzyme reversible inhibition effect, the total potato glycoalkaloids content can be determined within the range of 0.