Publications by authors named "Valentyna Kryklyva"
Purpose: This study aims to introduce an innovative multi-step pipeline for automatic tumor-stroma ratio (TSR) quantification as a potential prognostic marker for pancreatic cancer, addressing the limitations of existing staging systems and the lack of commonly used prognostic biomarkers.
Methods: The proposed approach involves a deep-learning-based method for the automatic segmentation of tumor epithelial cells, tumor bulk, and stroma from whole-slide images (WSIs). Models were trained using five-fold cross-validation and evaluated on an independent external test set.
Article Synopsis
- * Researchers analyzed DNA from invasive pancreatic adenocarcinomas and precursor lesions of patients with and without ATM genetic variants to assess how these alterations contribute to cancer development.
- * Findings revealed that somatic ATM alterations were present in a significant majority of invasive cancers (75%) but were much less common in precursor lesions (7.1%), suggesting that alterations may occur later in the progression of pancreatic cancer.*
Mismatch repair deficiency (dMMR) is a hallmark of Lynch syndrome (LS), but its prevalence in early-onset (diagnosed under the age of 50 years) duodenal, ampullary, and pancreatic carcinomas (DC, AC, and PC, respectively) is largely unknown. We explored the prevalence of dMMR and the underlying molecular mechanisms in a retrospectively collected cohort of 90 early-onset carcinomas of duodenal, ampullary, and pancreatic origin. dMMR was most prevalent in early-onset DCs (47.
View Article and Find Full Text PDFTumor mutational burden (TMB) is a numeric index that expresses the number of mutations per megabase (muts/Mb) harbored by tumor cells in a neoplasm. TMB can be determined using different approaches based on next-generation sequencing. In the case of high values, it indicates a potential response to immunotherapy.
View Article and Find Full Text PDFMedullary pancreatic carcinoma (MPC) is a rare histological variant of pancreatic ductal adenocarcinoma (PDAC). Because of its rarity, data on the molecular background of MPC are limited. Previous studies have shown that a subset of MPCs is microsatellite instable due to mismatch repair deficiency.
View Article and Find Full Text PDFArticle Synopsis
- The study focuses on pancreatic ductal adenocarcinoma (PDAC) with microsatellite instability (MSI) or defective DNA mismatch repair (dMMR), highlighting the growing interest in immunotherapy for this type of tumor.
- A systematic review included 34 studies involving over 8,000 patients, revealing that MSI/dMMR is rare in PDAC (1%-2%) and is linked to specific histological subtypes like medullary and mucinous/colloid variants.
- The conclusion emphasizes the importance of routinely testing for MSI/dMMR in PDAC cases with these histologies, recommending specific testing methods and next-generation sequencing for better precision in treatment.
Acinar cell carcinoma (ACC) is a rare pancreatic neoplasm with dismal prognosis. Insights into the molecular basis of ACC can pave the way for the application of more effective, personalized therapies and detection of patients with hereditary predisposition. Molecular analysis revealed a germline (and ) mutation in a patient with a rare pancreatic ACC with extensive intraductal growth.
View Article and Find Full Text PDFInvasive cancer cells form actin-rich membrane protrusions called invadopodia that degrade extracellular matrix and facilitate cell invasion and metastasis. WIP (WASP-interacting protein) together with N-WASP (neural Wiskott-Aldrich syndrome protein) are localized in invadopodia and play a crucial role in their formation. Here we show that WIP interacts with endocytic adaptor proteins of the intersectin (ITSN) family, ITSN1 and ITSN2.
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