Publications by authors named "Valentina Gasca"
Although every cell biologist knows the importance of selecting the right growth conditions and it is well known that the composition of growth medium may vary depending on a product brand or lot affecting many cellular processes, still those effects are poorly systematized. We addressed this issue by comparing the effect of 12 fetal bovine sera (FBS) and eight growth media from different brands on the morphological and functional parameters of five cell types: lung adenocarcinoma, neuroblastoma, glioblastoma, embryonic kidney, and colorectal cancer cells. Using high-throughput imaging, we compared cell proliferation; performed morphological profiling based on the imaging of 561,519 cells; measured extracellular regulated kinases (ERK1/2) activity, mitochondria potential, and lysosome accumulation; and compared cell sensitivity to drugs, response to EGF stimulation, and ability to differentiate.
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- Imaging-based anticancer drug screening is gaining traction due to advancements in fluorescence microscopy and image processing, enabling detailed study of cell viability and proliferation.
- Despite the availability of large-scale datasets for cell viability, the integration of toxicology measurements from these screens with imaging data remains uncertain.
- This research shows that imaging methods yield high accuracy in assessing drug effects across various cancer cell lines and that selecting specific analysis metrics can improve consistency in results while highlighting the benefits of monitoring mitochondria and cell dynamics.