Publications by authors named "Valentina Fateeva"
Primary cultures of rat hepatocytes grown on slides were studied in serum-free medium. Ultradian protein synthesis rhythm was used as a marker of synchronization of individual oscillations, resulting in the formation of a common rhythm of the cell population, i.e.
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- Ultradian oscillations in protein synthesis indicate how hepatocytes (liver cells) can work together rhythmically, with stronger rhythms linked to better cell cooperation.
- Experiments with different cultures of young and old rat hepatocytes showed younger cells generally had more pronounced rhythms, but environmental factors like the medium composition can significantly impact the results.
- The addition of specific substances like gangliosides or phenylephrine could enhance the protein synthesis rhythms in older cells to levels seen in younger ones, suggesting that the cell environment plays a crucial role in cooperation more than just age.
Article Synopsis
- The study investigates the role of ultradian protein synthesis rhythms as indicators of cooperation among liver cells (hepatocytes) in different culture conditions.
- Phenylephrine and a specific compound (2,5-di(tertiary-butyl)-1,4-benzohydroquinone) were found to stimulate cooperative behavior in sparse cultures by increasing intracellular calcium levels ([Ca(2+)](cyt)).
- The experiment shows that disrupting calcium signaling with BAPTA-AM abolished the protein synthesis rhythm, highlighting the importance of calcium and gangliosides (like GM1) in synchronizing cell activities and modulating protein metabolism.
Pretreatment of hepatocyte cultures with 1 microM d-l-threo-1-phenyl-2-hexadecanoylamino-3-pyrrolidino-1-propanol-HCL (PPPP) for 24 h decreased the ganglioside GM1 content of the cells by approximately 50% and that of the conditioned medium by 90%. No rhythm in the rate of protein synthesis was detected in dense cultures pretreated with PPPP, but was observed in control dense cultures. Conditioned medium from control dense cultures induced synchrony in sparse cultures, which were non-synchronous in their own medium.
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