Identification of the novel HLA-B*51:367 allele in a cord blood donor by next-generation sequencing.

The new HLA-B*51:367 differs from B*51:01:01:64 by four substitutions in exon 1.

Characterization of the novel HLA-DPA1 allele, HLA-DPA1*01:86.

HLA-DPA1*01:86 differs from DPA1*01:03:01:05 by a single nucleotide change in codon 94 (AAG>GAG).

Use of DPB1 T-cell epitope algorithm among italian transplant centers: A survey on behalf of Associazione Italiana di Immunogenetica e Biologia dei Trapianti.

The HLA-DPB1 locus has been demonstrated to have a significant role on patients' outcome after allogeneic HSCT, and the so-called T-cell epitope (TCE) algorithm has been incorporated in international guidelines for the selection of unrelated donors. The purpose of the present study is to measure, through a national survey conducted on behalf of the Associazione Italiana di Immunogenetica e Biologia dei Trapianti (AIBT), the extent of awareness and use of HLA-DPB1 TCE-based algorithms during the donor search. 89% of the HLA laboratories answered to a short questionnaire and the results showed a progressive increase of the laboratories typing DPB1 in patients and their potential donors during the search (from 44% to 79% during the 2010-2019 period) as well as the application of a TCE-based algorithm for the donor choice whenever possible (from 24% to 65% during the same period).

Identification of a new HLA-A allele: A*31:187.

HLA-A*31:187 differs from A*31:01:02:04 by a single nucleotide change in codon 80 (ACC > AAC).

Identification of a single nucleotide deletion in the novel HLA-DQB1*06:379N allele, detected by Polymerase Chain Reaction-Sequence Based Typing but not by Next Generation Sequencing.

In this report we describe the characterization of a novel null HLA-DQB1 allele, detected by polymerase chain reaction-sequence-based typing but not by next-generation sequencing (NGS). The new allele, HLA-DQB1*06:379N, was discovered in an Italian patient with acute myeloid leukemia and also in one of her healthy siblings. The new allele is largely homologous to DQB1*06:02:01:01 with a T deletion in exon 2, in codon 11, which causes a frameshift and the formation of an unusual and premature TGA STOP in codon 27.

The novel HLA-A*68 variant, HLA-A*68:02:16, identified by next-generation sequencing.

HLA-A*68:02:16 differs from A*68:02:01:01 by a single synonymous nucleotide change in codon 160 (CTG>CTA).

Characterization of the novel HLA-DPA1 allele, HLA-DPA1*01:27.

HLA-DPA1*01:27 differs from DPA1*01:03:01:01 by a single non-synonymous nucleotide change in codon 15 (ACG > ATG).