Disentangling the metabolic profile of rumen ciliates: A historical perspective and future directions.

Rumen ciliates constitute a highly diverse group within the subclass Trichostomatia. They are known for their symbiotic relationship with ruminant hosts and their pivotal role in digestive metabolism. This review presents a historical analysis of research on rumen ciliate metabolism, since the earliest studies, based on in vitro and in situ experiments, as well as molecular studies, initially relying on Sanger sequencing, and more recently, next-generation sequencing techniques.