Publications by authors named "Valber Albuquerque Pedrosa"
Article Synopsis
- - The study introduces an affordable and user-friendly colorimetric kit for diagnosing porcine circovirus 2 (PCV-2), which can be interpreted without special equipment, using hybrid nanoparticles linked with specific antibodies for the virus.
- - Testing involved 40 positive samples confirmed by qPCR and 10 negative samples, with interference tests against other viruses to ensure accuracy; a noticeable color change (purple for positive and red for negative) occurs within about a minute after sample application.
- - This diagnostic method uses techniques like localized surface plasmon resonance (LSPR) and transmission electron microscopy (TEM) for validation and presents a cost-effective alternative to traditional methods, with a kit price of only USD 4.00.
Researchers worldwide have been studying alternatives to detect SARS-CoV-2 (COVID-19), and accurate and timely diagnosis is crucial for controlling the outbreaks of the disease. Surface plasmon resonance (SPR) is an effective strategy based on antibodies, and it can be used for simple and fast detection of antibodies due to COVID-19 infection. Accordingly, this paper reports on the highly sensitive and specific detection of antibody responses to SARS-CoV-2 spike (S) and nucleocapsid (N) proteins in COVID-19 patients.
View Article and Find Full Text PDFGold nanoparticles (AuNPs) can provide a simple, easy-to-use, inexpensive, at hand point-of-care (POC) fast to diagnose; however, AuNPs have the predisposition to form aggregations. Since the nanoparticles stability is an important issue, this article is aiming to study the long-term stability associated with the development of an immunosensor for clinical diagnosis. Here, we assessed two previous methods commonly described in the literature to prevent the formation of aggregate by studying pH and Tween® 20 (polysorbates) addition as surfactant.
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