Quantification of polyketide synthase genes in tropical urban soils using real-time PCR.

Polyketide synthases (PKSs) catalyze the biosynthesis of polyketides and may contribute to the natural production of antibiotics and pose selective pressure for the development of antibiotic resistant bacteria in the environment. Although conventional PCR have been developed to detect the presence of PKS genes, no previous studies have been done to quantify the abundance of PKS genes in environmental samples. In this study, two sets of degenerate real-time PCR (qPCR) primers (PKS1-F/PKS1-R, PKS2-F/PKS2-R) with high specificity and sensitivity were developed to quantify PKS type I and type II genes.