Controlled Environment Ecosystem: A Cutting-Edge Technology in Speed Breeding.

The controlled environment ecosystem is a meticulously designed plant growing chamber utilized for cultivating biofortified crops and microgreens, addressing hidden hunger and malnutrition prevalent in the growing population. The integration of speed breeding within such controlled environments effectively eradicates morphological disruptions encountered in traditional breeding methods such as inbreeding depression, male sterility, self-incompatibility, embryo abortion, and other unsuccessful attempts. In contrast to the unpredictable climate conditions that often prolong breeding cycles to 10-15 years in traditional breeding and 4-5 years in transgenic breeding within open ecosystems, speed breeding techniques expedite the achievement of breeding objectives and F1-F6 generations within 2-3 years under controlled growing conditions.

Production of, Factors Affecting, Gene Regulations, and Challenges in Tissue Cultured Plant through Soilless Culture.

Soilless culture also known as water based culture and substrate based culture has immense potential to grow tissue cultured plants in a closed and controlled environment system. This review analyzes the various factors that affect the vegetative growth, reproductive growth, metabolic processes, and gene regulatory functions of tissue cultured plants and the suitability of soilless culture for tissue culture plants. Experiments show that morphological and reproductive abnormalities are mitigated in tissue cultured plants by gene regulation in a closed and controlled environment system.