Micronucleus assay in bovine lymphocytes after exposure to bisphenol A in vitro.

Bisphenol A (BPA) [2,2-bis-(4-hydroxyphenyl) propane] is an important industrial agent, made by combining acetone and phenol, that is used extensively as a monomer in the production of polycarbonate plastics and as a precursor of epoxy resins. Micronucleus assays have served as an index of cytogenetic damage in in vivo and in vitro studies. We studied the genotoxic and cytotoxic effects of BPA on bovine peripheral lymphocytes in vitro.

Chromosomal aberrations in ovine lymphocytes exposed in vitro to tolylfluanid.

Chromosomal aberrations have been used as important cytogenetic biomarkers to study the mutagenic effects of different chemicals in vivo and in vitro. Chromosomal aberrations were evaluated in cultures of sheep lymphocytes in vitro exposed to the fungicide tolylfluanid. Lymphocyte cultures from three donors were exposed to four different concentrations of fungicide (1.

Micronucleus frequency in sheep lymphocytes after in vitro exposure to fungicide tolylfluanid.

The fungicide tolylfluanid (N -dichlorofluoromethylthio-N', N -dimethyl -N -p -tolylsulfamide), was investigated by cytokinesis-block micronucleus assay. Tolylfluanid at the lowest concentration (1 x 10(- 6)mol L(- 1))did not influence significantly the frequency of micronuclei in sheep lymphocyte cultures in comparison with control (32.33 +/- 3.

Some food toxic for pets.

According to world statistics, dogs and cats are the species that owners most frequently seek assistance with potential poisonings, accounting 95-98% of all reported animal cases. Exposures occur more commonly in the summer and in December that is associated with the holiday season. The majority (>90%) of animal poisonings are accidental and acute in nature and occur near or at the animal owner's home.

Chromosomal aberrations and frequency of micronuclei in sheep subchronically exposed to the fungicide Euparen Multi (tolylfluanid).

We analyzed chromosome aberrations, micronucleus frequency, mitotic index (MI), and nuclear division index (NDI) in peripheral lymphocytes of sheep subchronically exposed to the fungicide Euparen Multi (containing 50% tolylfluanid). Euparen Multi was administered by rumen sonde to group of Merino sheep (seven sheep/group) at 93 mg/kg body weight (1/20 LD50) daily for 28 days to assess its genotoxic effects. The frequencies of aberrant cells (ABC) in the experimental and control groups were 5.

Chromosome damage in peripheral lymphocytes of sheep induced by chlorine in drinking water.

The potential of chromosomal damage associated with the effects of chlorine in drinking water was evaluated using chromosome aberrations and micronuclei as cytogenetic endpoints in the lymphocytes of peripheral blood of ewe lambs. The study assessed the in vivo effects of high chlorine doses (1.8 mg l(-1), based on Savo-Super disinfectant) in drinking water on the peripheral lymphocytes of sheep after 30 days.

A possible relationship between viral infection and chromosome damage in breeding boars.

A cytogenetic analysis of peripheral blood lymphocytes was carried out on breeding boars (n = 5) on a farm where viral infections (porcine parvovirosis) had occurred. Several different types of chromosomal aberrations were observed including chromatid breaks (23.60 +/- 2.