Comparative Whole-Transcriptome Profiling of Liver Tissue from Wistar Rats Fed with Diets Containing Different Amounts of Fat, Fructose, and Cholesterol.

Differential expression of 30,003 genes was studied in the liver of female Wistar rats fed with isocaloric diets with the excess of fat, fructose, or cholesterol, or their combinations for 62 days using the method of whole-transcriptome profiling on a microchip. Relative mRNA expression levels of the Asah2, Crot, Crtc2, Fmo3, GSTA2, LOC1009122026, LOC102551184, NpY, NqO1, Prom1, Retsat, RGD1305464, Tmem104, and Whsc1 genes were also determined by RT-qPCR. All the tested diets affected differently the key metabolic pathways (KEGGs).

Transcription profile analysis of the endometrium revealed molecular markers of the personalized 'window of implantation' during in vitro fertilization.

To determine the most informative markers for assessing the functional state of endometrium during the 'window of implantation' and creating a model for assessment of the readiness of endometrium for embryo implantation. Forty-seven women with tubal infertility and a successful IVF pregnancy participated in the study. Pipelle endometrial sample was performed during the supposed 'window of implantation' in natural cycle with subsequent histological study, and transcriptional profile of genes GPX3, PAEP, DPP4, TAGLN, HABP2, IMPA2, AQP3, HLA-DOB, MSX1, POSTN determined by real-time quantitative polymerase chain reaction (qRT-PCR).

Method of Selection of Bacteria Antibiotic Resistance Genes Based on Clustering of Similar Nucleotide Sequences.

A new method for selection of bacterium antibiotic resistance genes is proposed and tested for solving the problems related to selection of primers for PCR assay. The method implies clustering of similar nucleotide sequences and selection of group primers for all genes of each cluster. Clustering of resistance genes for six groups of antibiotics (aminoglycosides, β-lactams, fluoroquinolones, glycopeptides, macrolides and lincosamides, and fusidic acid) was performed.

An integrative analysis of reprogramming in human isogenic system identified a clone selection criterion.

The pluripotency of newly developed human induced pluripotent stem cells (iPSCs) is usually characterized by physiological parameters; i.e., by their ability to maintain the undifferentiated state and to differentiate into derivatives of the 3 germ layers.

Genome-wide association study identifies three novel genetic markers associated with elite endurance performance.

To investigate the association between multiple single-nucleotide polymorphisms (SNPs), aerobic performance and elite endurance athlete status in Russians. By using GWAS approach, we examined the association between 1,140,419 SNPs and relative maximal oxygen consumption rate ([Formula: see text]O2max) in 80 international-level Russian endurance athletes (46 males and 34 females). To validate obtained results, we further performed case-control studies by comparing the frequencies of the most significant SNPs (with P < 10(-5)-10(-8)) between 218 endurance athletes and opposite cohorts (192 Russian controls, 1367 European controls, and 230 Russian power athletes).

AGTR2 gene polymorphism is associated with muscle fibre composition, athletic status and aerobic performance.

Muscle fibre type is a heritable trait and can partly predict athletic success. It has been proposed that polymorphisms of genes involved in the regulation of muscle fibre characteristics may predispose the muscle precursor cells of a given individual to be predominantly fast or slow. In the present study, we examined the association between 15 candidate gene polymorphisms and muscle fibre type composition of the vastus lateralis muscle in 55 physically active, healthy men.

SOD2 gene polymorphism and muscle damage markers in elite athletes.

Exercise-induced oxidative stress is a state that primarily occurs in athletes involved in high-intensity sports when pro-oxidants overwhelm the antioxidant defense system to oxidize proteins, lipids, and nucleic acids. During exercise, oxidative stress is linked to muscle metabolism and muscle damage, because exercise increases free radical production. The T allele of the Ala16Val (rs4880 C/T) polymorphism in the mitochondrial superoxide dismutase 2 (SOD2) gene has been reported to reduce SOD2 efficiency against oxidative stress.

Associations of single nucleotide polymorphisms with malignant and borderline bone tumors.

Bone neoplasms - are a rare group of diseases, which ethiology and pathogenesis are not fully understood. We have studied 6 single nucleotide polymorphisms rs792/(GHI), rs7956547(IGFI), rs3761243(GNRH2), rs11737764(FGF2), rs6599400(FGFR3), and rs1690916(MDM2) associations with bone tumors. In our work we've detected significant associations with some single nucleotide polymorphisms: IGFl.

Genome-scale analysis of DNA methylation in colorectal cancer using Infinium HumanMethylation450 BeadChips.

Illumina's Infinium HumanMethylation450 BeadChip arrays were used to examine genome-wide DNA methylation profiles in 22 sample pairs from colorectal cancer (CRC) and adjacent tissues and 19 colon tissue samples from cancer-free donors. We show that the methylation profiles of tumors and healthy tissue samples can be clearly distinguished from one another and that the main source of methylation variability is associated with disease status. We used different statistical approaches to evaluate the methylation data.

Association of FGFR3 and MDM2 gene nucleotide polymorphisms with bone tumors.

Association study of 6 candidate single-nucleotide polymorphisms (rs7921, rs7956547, rs3761243, rs11737764, rs6599400, rs1690916) was carried out in a group of patients with bone tumors of different histological structure (n=68) and control group of normal subjects (n=96). Significant associations of rs6599400 and rs1690916 polymorphisms with disease risk were detected (odds ratio 2.15 [1.

Comparison of the Level of DNA-Hydrolyzing Polyclonal IgG Antibodies in Sera of Patients with Hashimoto's Thyroiditis and Nontoxic Nodal Goiter.

Various catalytically active IgG antibodies or abzymes have been recently detected in the sera of patients with several autoimmune pathologies, where their presence is most probably associated with autoimmunization. Here we show for the first time that IgG from peripheral blood of patients with Hashimoto's thyroiditis and nontoxic nodal goiter possesses DNase activity. The properties of the DNase IgGs distinguished them from other known human DNases.

Variability of Substrate Specificity of Serum Antibodies Obtained from Patients with Different Autoimmune and Viral Diseases in Reaction of tRNA Hydrolysis.

Recently we have shown that the substrate specificity of catalytic IgG isolated from sera of patients with Hashimoto's thyroiditis, systemic lupus erythematosus (SLE), polyarthritis and hepatitis B for classic poly(N) homopolynucleotide substrates and for specific tRNA(Phe) with compact and stable structure was correlated with the type of disease. At the same time the cleavage specificity was different in comparison with that of all known human RNases. Here we investigated for the first time the hydrolysis by the IgGs isolated from sera of 31 patients with different diseases of the in vitro transcript of human mitochondrial tRNA(Lys) which has less stable structure as compared to tRNA(Phe).

[Some real problems of human opisthorchiasis].

Forty six sera from residents of the Novosibirsk Region in whom the diagnosis of chronic opisthorchiasis had been helminthoovoscopically verified were examined. In the thin layer immunoassay, of them 14 (30.4%) sera were responsive to excretory O.

Catalytic diversity of polyclonal RNA-hydrolyzing IgG antibodies from the sera of patients with systemic lupus erythematosus.

Various catalytic antibodies or abzymes have been detected in the sera of patients with several autoimmune pathologies, and recently we have shown that RNase activity is associated with IgGs and IgMs from the sera of patients with systemic lupus erythematosus (SLE) but not with those from the sera of normal humans. Here we present for the first time convincing evidence showing that highly purified SLE IgG, its F(ab) fragments and separated L-chains catalyze RNA hydrolysis. These IgGs hydrolyze RNA about one to three orders of magnitude faster than DNA.

[Comparative analysis of methods of diagnosis of chlamydia infection].

In case of a correct sampling, the diagnostic value of optic and electron microscopy for detecting Chlamydia infection is not inferior to that of direct microimmunofluorescence (DMIF) and higher than that of enzyme immunoassay (EIA). Optic microscopy showed that basal vaginal epithelium and buccal mucosa can be infected with Chlamydia. Provazek bodies were detected in the buccal mucosa of the overwhelming majority of patients with genital chlamydiasis.

Effect of different drugs on the level of DNA-hydrolyzing polyclonal IgG antibodies in sera of patients with Hashimoto's thyroiditis and nontoxic nodal goiter.

Background: DNA-hydrolyzing IgG antibodies have been detected recently in the sera of patients with several autoimmune diseases.

Material And Methods: The relative activity of DNA-hydrolyzing IgG from the sera of patients with Hashimoto's thyroiditis and with non-toxic nodal goiter as a function of the patient's condition were measured. The effect of different drugs on the level of DNA-hydrolyzing IgG, functional activity of thyroid gland, and improvement of clinical condition of two groups of patients were analyzed.

Catalytic heterogenity of polyclonal RNA-hydrolyzing IgM from sera of patients with lupus erythematosus.

Various catalytically active IgG antibodies or abzymes have been detected recently in the sera of patients with several autoimmune pathologies including systemic lupus erythematosus (SLE), where their presence is most probably associated with autoimmunization. Here we show for the first time that IgM from peripheral blood of patients with SLE possesses both DNase and RNase activities: these activities were also present in Fab fragments of the IgM. Both specific enzymic activities of IgM from sera of any single patient are usually 5-10 times higher than those of IgG antibodies.

Methorchiasis in the residents of Novosibirsk area, Russia.

Infection with Methorchis bilis was recognized for the first time in the residents of Novosibirsk area (Russia). During a serological survey (37 patients in toto), it was possible to demonstrate that 48.5% of the serum samples tested possessed antibodies to Opisthorchis felineus antigens, 37.

[Development of a two-site immunoassay using polyclonal antibodies for determination of lactoferrin in human blood].

The two-site enzyme-linked immunosorbent assay (ELISA) for lactoferrin using polyclonal antibodies to spatially distant epitopes has been developed. The assay sensitivity defined as minimal detectable lactoferrin concentration for p = 0.05 is 0.

Polyclonal antibodies from blood and cerebrospinal fluid of patients with multiple sclerosis effectively hydrolyze DNA and RNA.

It is known that in the blood of patients with some autoimmune diseases catalytically active antibodies hydrolyzing proteins, DNA, and RNA may be detected. In the present work homogeneous preparations of IgG antibodies (Ab) possessing high affinity for nucleic acids (NA) were obtained for the first time from blood and cerebrospinal fluid of patients with multiple sclerosis (MS). The fraction of IgG Ab as well as its Fab fragments and isolated light chains of both kappa- and lambda-types were shown to catalyze effectively the hydrolysis of DNA and RNA.

DNA- and RNA-hydrolyzing antibodies from the blood of patients with various forms of viral hepatitis.

Antibodies (Abs) hydrolyzing proteins, DNA, and RNA are detected in the blood of patients with various autoimmune diseases. In the present work, homogeneous preparations of IgG Abs from the blood of the healthy donors as well as patients with A, B, C, and delta types of viral hepatitis, influenza, pneumonia, tuberculosis, tonsillitis, duodenal ulcer, and some types of cancer were purified. For the first time, the fraction of IgG and its Fab fragments of patients with viral hepatitis were shown to have high DNA- and RNA-hydrolyzing activity.

RNA-hydrolyzing antibodies from peripheral blood of patients with lupus erythematosus.

Experiments and hydrolysis of substrates with known spatial structures (such as yeast tRNAPhe, as well as normal and mutant tRNALys from human mitochondria produced by transcription of the appropriate DNA species, that is, RNA genes) were performed to study the ribonuclease activity of antibodies isolated from blood sera of patients with systemic lupus erythematosus (SLE). The antibody preparations contained two types of ribonuclease activities: the first corresponded to the specificity of ribonuclease A and was found during hydrolysis at low salt concentrations, whereas the second was stimulated by Mg2+ and displayed unique specificity toward double-stranded regions of the substrate. The possible use of the antibody preparations as tools for structural studies of conformational differences between RNA molecules was examined.