An optimized permeabilization step for flow cytometry analysis of nuclear proteins in myeloid differentiation of blood cells into neutrophils.

Polymorphonuclear leukocytes (PMNLs) or neutrophils play an important role in the innate immune response. Working with human neutrophils is challenging because these cells are sensitive to changes in the surrounding media and quickly become apoptotic. Meanwhile the experiments with mature neutrophils may be very important for studies of blood function.

[New alkyl cationic glycerolipids with heterocyclic polar domain are responsible for disorders in the leukemia cells cycle and cells apoptosis].

A series of new modifications of alkyl cationic glycerolipids with hetero polar domain has been synthesized. The most active compound rac-N-(4-[(2-ethoxy-3-octadecyloxy)prop-1-yloxycarbonyl]butyl)-N'-etylimidazoliyiodid in micromolar concentrations causes a delay of cell cycle in phase G1, DNA fragmentation and apoptosis of cell line leukemia.

Oceanographic lidar attenuation coefficients and signal fluctuations measured from a ship in the Southern California Bight.

We measured the attenuation coefficient of the National Oceanic and Atmospheric Administration lidar from a ship in the Southern California Bight in September 1995. The region from approximately 5 to 30 m in depth was covered. The laser was linearly polarized, and the receiver was operated with the same polarization and the orthogonal polarization.

Lidar profiles of fish schools.

A lidar system was used in a seawater tank to measure the average diffuse reflectivity of live sardines. Diffuse reflectivity was measured to be 10 % for a copolarized laser return and 3 % for a cross-polarized return. We used these calibration measurements to infer the density of sardines in areas of the Southern California Bight from vertical profiles obtained with the lidar mounted on a ship.