[Effect of a high-frequency electrical current on the activity of the raphe nuclei and the locus coeruleus].

Recovery cycles of primary evoked potentials to light flashes in the visual cortical area of waking rats were studied under conditions of pharmacological and electrical influences on serotonin (5-HT)- and noradren (NA)ergic brain systems. All factors used induced oscillations of the recovery cycles. Periods of oscillations were similar (300-400 ms) during pharmacological suppression of the NA-system and during high-frequency (500 Hz) electrical stimulation or lesion of locus coeruleus.

[Changes in regional hemodynamics and gas exchange in healthy males after moderate blood loss and blood reinfusion in response to antiorthostatic hypokinesia].

Nine healthy male test subjects were exposed for 7 days to head-down tilting. Within 2 hours after exposure 500 ml of blood were withdrawn. This reduced pulse blood filling of all lung compartments, particularly upper (P less than 0.

[Intensive psychopharmacologic therapy of slowly-progressive schizophrenia].

The authors analyze the results of psychopharmacotherapy of 120 patients with slowly progressive schizophrenia characterized by a high resistance to the treatment. Two new methods of the intensive therapy of such conditions (discrete and daily intravenous drip of tranquilizers in conjunction with low doses of neuroleptics) are described. The pharmacological and pharmacokinetic substantiation of each of the two techniques is presented.

Cortisone-induced small RNP tightly bound to chromatin.

The small nuclear RNP (alpha-RNP) tightly bound to chromatin has been isolated. alpha-RNP can be removed from chromatin together with the acid-soluble proteins. The RNA from this RNP has been isolated; its electrophoretic mobility is equal to that of 4 S RNA.

[Effect of 120-day antiorthostatic hypokinesia on gas exchange and pulmonary circulation in humans].

Parameters of gas exchange and pulmonary circulation were measured in five healthy test subjects during 120-day head-down tilt test and early recovery. During the first half of the bed rest study CO2 tension in arterial blood increased significantly. During the second half of the study oxygen and carbon dioxide tension decreased significantly.

[Copper and manganese metabolism during the administration of therapeutic doses of iron].

Examination of normal men and women with the aid of emission spectral analysis has shown that high muscular training led to the intensification of the retention of iron additionally administered to the body. This indicates concealed deficiency of this element in the body. Administration of therapeutic doses of iron essentially raised copper and manganese excretion from the body.

[Ultrastructure of the cerebral cortex of the cat in the early recovery period after short-term anoxia].

The ultrastructure of cat sensomotor cortex has been studied during a 15-minute recovery after 2.5-6-minute oxygen supply cessation. An increase of osmopholia of free and endoplasmic reticulum bound ribosomes was detected in addition to a great number of altered mitochondria with longitudinal crystal arrangement.

[Effect of head-down-tilt hypokinesia on pulmonary blood flow and gas exchange].

The purpose of the study was to investigate gas exchange and lung perfusion during 14-day head-down tilt and immediately thereafter. During head-down tilting pulmonary circulation increased, as suggested by zonal rheography of the lungs and by ECG (increase in the PII-III amplitude and width by 1-2 mm and 0.01-0.

[Processes of reconstruction and association by nucleosomes with various formations of histones].

The experiments on reconstruction of chromatin (without H1) from DNA and histone octamer containing either H2B from sea urchin sperm (H2B-S) or H2B from calf thymus are reported. It has been shown that H2B-S affects the mode of interaction of histones with DNA during the reconstitution of nucleosomal particles on one hand and on the other hand H2B-S plays a major role in the interactions of reconstituted mononucleosomes. These interactions result in supranucleosomal structures.

[Conformational properties of protamines].

Conformational peculiarities of protamines in a wide range of various conditions have been studied by the CD and X-ray analysis. Characteristic peculiarities of CD-spectra and position of diffraction maximum in X-ray diagrams of protamines argue in favor that their conformation is similar to that of the extended left handed helix of poly(L)-proline II. Though conformation parameters of this structure are very sensitive to temperature or pH variations, to specific action of different ions, alcohols, and detergents the structure itself is rather stable and is destroyed under extreme conditions only.

[Comparative study of synthetic peptide fragments of histones H2B].

Oligopeptides of the N-terminal end of H2B histone with different molecular weights and amino acid sequences are studied by the CD method and X-ray analysis. Characteristic peculiarities of CD-spectra and position of diffraction maximums in X-ray diagrams in all the studied oligopeptides show that their conformation by the structure is similar to the extended left--handed helix of polyproline II. The investigation of CD-spectra under different conditions showed the dependence of the stability of "proII" conformation on the oligopeptide chain length.

The role of histone H2B from sea urchin sperm in the association of reconstituted minichromosomes.

A comparative study of the condensation of reconstituted complexes of circular SV40 DNA with core histones from calf thymus and sea urchin sperm was performed using sedimentation and electron microscopic techniques. It is shown that in low ionic strength solutions both types of complexes are similar to native 'minichromosomes'. In the region from 0.

[Steroid-sensitive neurons of the rabbit and mouse hippocampus].

On the whole brain of rabbits and surviving slices of the hippocamp of mice, studies have been made of the sensitivity and peculiarities of the reaction of hippocampal neurones to hydrocortison administration. In experiments on whole animals, the drug was applied to the hippocampal neurones by means of microionophoretic technique. In experiments with slices, the steroid hormone was injected with a special micropipette directly in to the chamber with the slice.