Spaceflight causes strain dependent gene expression changes associated with lipid and extracellular matrix dysregulation in the mouse kidney in vivo.

To explore new worlds we must ensure humans can survive and thrive in the space environment. Incidence of kidney stones in astronauts is a major risk factor associated with long term missions, caused by increased blood calcium levels due to bone demineralisation triggered by microgravity and space radiation. Transcriptomic changes have been observed in other tissues during spaceflight, including the kidney.

Problem resolution scale: A single-item instrument for easily assessing clinical improvement.

This study describes the development and tests the validity of the Problem Resolution Scale (PRS)-a single-item measure developed by researchers at Systemic Practice Research Network (SYPRENE) for assessing the degree to which the focal problem of therapy is perceived as resolved. Data were collected at termination from 747 clients seen across 18 therapists. Results suggested good construct validity, supported by a strong correlation between client and therapist perceptions as assessed by the PRS (r = 0.

Muscle atrophy phenotype gene expression during spaceflight is linked to a metabolic crosstalk in both the liver and the muscle in mice.

Human expansion in space is hampered by the physiological risks of spaceflight. The muscle and the liver are among the most affected tissues during spaceflight and their relationships in response to space exposure have never been studied. We compared the transcriptome response of liver and quadriceps from mice on NASA RR1 mission, after 37 days of exposure to spaceflight using GSEA, ORA, and sparse partial least square-differential analysis.

Potential for inhibition of checkpoint kinases 1/2 in pulmonary fibrosis and secondary pulmonary hypertension.

Background: Idiopathic pulmonary fibrosis (IPF) is a chronic lung disease characterised by exuberant tissue remodelling and associated with high unmet medical needs. Outcomes are even worse when IPF results in secondary pulmonary hypertension (PH). Importantly, exaggerated resistance to cell death, excessive proliferation and enhanced synthetic capacity are key endophenotypes of both fibroblasts and pulmonary artery smooth muscle cells, suggesting shared molecular pathways.

Pt-ttpy, a G-quadruplex binding platinum complex, induces telomere dysfunction and G-rich regions DNA damage.

Pt-ttpy (tolyl terpyridin-Pt complex) covalently binds to G-quadruplex (G4) structures in vitro and to telomeres in cellulo via its Pt moiety. Here, we identified its targets in the human genome, in comparison to Pt-tpy, its derivative without G4 affinity, and cisplatin. Pt-ttpy, but not Pt-tpy, induces the release of the shelterin protein TRF2 from telomeres concomitantly to the formation of DNA damage foci at telomeres but also at other chromosomal locations.

Oxidized DNA Precursors Cleanup by NUDT1 Contributes to Vascular Remodeling in Pulmonary Arterial Hypertension.

Pulmonary arterial hypertension (PAH) is a life-threatening condition characterized by abnormally elevated pulmonary pressures and right ventricular failure. Excessive proliferation and resistance to apoptosis of pulmonary artery smooth muscle cells (PASMCs) is one of the most important drivers of vascular remodeling in PAH, for which available treatments have limited effectiveness. To gain insights into the mechanisms leading to the development of the disease and identify new actionable targets.

PIM1 (Moloney Murine Leukemia Provirus Integration Site) Inhibition Decreases the Nonhomologous End-Joining DNA Damage Repair Signaling Pathway in Pulmonary Hypertension.

Objective: Pulmonary arterial hypertension (PAH) is a fatal disease characterized by the narrowing of pulmonary arteries (PAs). It is now established that this phenotype is associated with enhanced PA smooth muscle cells (PASMCs) proliferation and suppressed apoptosis. This phenotype is sustained in part by the activation of several DNA repair pathways allowing PASMCs to survive despite the unfavorable environmental conditions.

Introducing SYPRENE: An International Practice Research Network for Strategic and Systemic Therapists and Researchers.

SYPRENE, a new international Systemic Practice Research Network (PRN), has been established to fill the gap in practice-based research on the effectiveness and efficiency of strategic therapies. This article presents the rationale for the creation of SYPRENE and describes data collection methods, and the encoding system implemented within this PRN. More developments are expected in the recruitment of practitioners, the types of data collected, findings, and the implementation of SYPRENE in supervision, trainings, and professional schools.

The cancer theory of pulmonary arterial hypertension.

Pulmonary arterial hypertension (PAH) remains a mysterious killer that, like cancer, is characterized by tremendous complexity. PAH development occurs under sustained and persistent environmental stress, such as inflammation, shear stress, pseudo-hypoxia, and more. After inducing an initial death of the endothelial cells, these environmental stresses contribute with time to the development of hyper-proliferative and apoptotic resistant clone of cells including pulmonary artery smooth muscle cells, fibroblasts, and even pulmonary artery endothelial cells allowing vascular remodeling and PAH development.

Implication of Inflammation and Epigenetic Readers in Coronary Artery Remodeling in Patients With Pulmonary Arterial Hypertension.

Objective: Pulmonary arterial hypertension (PAH) is a vascular disease not restricted to the lungs. Many signaling pathways described in PAH are also of importance in other vascular remodeling diseases, such as coronary artery disease (CAD). Intriguingly, CAD is 4× more prevalent in PAH compared with the global population, suggesting a link between these 2 diseases.

Influence of the morphological aspect of embryos obtained by in vitro fertilization on their implantation rate.

In a series of 500 transfers of embryos obtained by in vitro fertilization, we examined the implantation rate of 1356 embryos transferred in utero. The average implantation rate per embryo was 15.1% and remained relatively constant, regardless of the number of transferred embryos per patient.

[Incidence of the morphological aspect on the rate of implantation of embryos obtained by fertilization in vitro].

1350 embryos obtained by In Vitro Fertilization (IVF) have been examined with the reverse microscope before their transfer in utero. Some embryos are "morphologically normal" (34.7%); some others are atypical.

Nucleolar changes in human embryo during the pre-implantation stage. Activation of ribosomal genes during the nucleologenesis.

We studied the structural and functional organization of human embryo nucleoli during the pre-implantation stage from spare embryos obtained by in vitro fertilization. In human embryo nucleoli, structural modifications occur during the first cleavages. They lead to the constitution of a reticulated nucleolus from an initial structure called primary nucleolus.

[Study of the action of an elastin polypeptide on dermal connective tissue].

The study of the effect of an elastin polypeptide on the connective tissue of rats, in the course of skin regeneration, showed an increase in fibrogenesis and fibroblastic hyperplasia in treated animals. The difference between treated and control animals is preserved after stopping of treatment after 15 days.

[Estimation of the quality of embryos obtained during fertilization in vitro as a function of their morphology].

Embryo quality after IVF should allow provisions for their pregnancy potential. We have classified all the transferred embryos into 4 types according to morphological criteria and we have studied the implantation rate of the different types. The results show: That pregnancies have been obtained with the 4 types of embryos.

[Effects of the injection of kainic acid into the medial hypothalamus on the secretion of corticolipotropic hormones in the rat].

alpha-MSH secretion was significantly lowered in Rats after lesion of the mediobasal hypothalamus with microinjections of kainic acid. beta-Endorphin release after ether-stress was also reduced. In opposition, no significant change in basal and ether-stress ACTH secretion was observed.

[Cytological diagnosis of ruptured membranes (author's transl)].

The authors have attempted to perform a simple and reliable test to affirm the diagnosis of a ruptured membranes. Various methods of staining and histochemical techniques were used. They conclude that the classical method for sampling and the alcohol-ether fixative are the best methods.

[Ultrastructure of the rat neurohypophysis following electric stimulation of the supraoptic nuclei].

By means of electrical stimulation of the supraoptic nuclei, in a stereotaxic apparatus, partial or complete degranulation of the neurohypophysis is obtained, with often a continued presence of large vesicles; the number of microvesicles is always increased.