[An acellular vaccine from "Pseudomonas aeruginosa." I. Preparation and activity (author's transl)].

From a strain (72 V) of Pseudomonas aeruginosa, we have prepared an acellular vaccine P2, which showed a good efficiency against homologous experimental infection in mice. The extraction procedure is as follows: washed bacterial cells are suspended in 0,15 M NaCl and heated at 60 degrees centigrade for 1 hr; after centrifugation, the supernatant fluid is precipitated with one and five volumes of ethanol. This acellular vaccine possess the following properties: rapid efficiency (10 days) after a single or three inoculations of very small doses (ED50 = 0.

[Evolution of fecal microflora in a "heteroxenic" infant maintained in a plastic isolator and trial on decontamination by antibiotherapy (author's transl)].

This work deals with the bacteriological study of an immunodeficient infant, delivered by aseptic cesarean section, and subsequently maintained in a plastic isolator. After a short period of germfree maintenance, several strains of bacteria were fortuitously introduced into the isolator, and became established in the infants' gastrointestinal tract. The qualitative and quantitative evolution of the fecal flora of this "heteroxenic" infant was followed for 170 days.

[Kinetics of setting up of a human fecal flora in germ free mice and trial of decontamination of antibiotherapy (author's transl)].

We have tried in this study to reproduce in germfree mice a bacterial equilibrium observed previously in the gastrointestinal tract of child "L.N.", reared in a plastic isolator.

[Nutrition and taxonomy of "enterobacteriaceae" and related bacteria. III. Nutritional characters and differentiation of the taxonomic groups (author's transl)].

A batch of 186 strains belonging to the families Enterobacteriaceae or Vibrionaceae has been studied by determination, for each strain, of the "versatility" towards 146 organic substrates tested as sole source of carbon and energy. This study allowed to work out a classification of these strains into the 32 classes which have been previously described. In the present paper the nutritional characters of these classes are reported.

[Nutrition and taxonomy of "enterobacteriaceae" and related bacteria. II. General results and classification (author's transl)].

A study of 186 strains belonging to eleven genera of the family Enterobacteriaceae and to three genera of the family Vibrionaceae has been carried out in order to determine their "versatility" towards 146 organic substrates tested as sole source of carbon and energy. Glucose was the only substrate used by all the strains; gluconate and glycerol were used by respectively 184 and 185 strains; 55 substrates were used by no one strains. The 90 substrates which were used by a fraction of the strains have served to establish a numerical classification of our strains, exclusively relied on these nutritional characters.

[Nutrition and taxonomy of "Enterobacteriaceae" and related bacteria. I. Technical procedure for auxanograms (author's transl)].

Study of nutritional capacities of 186 strains of Enterobacteriaceae or related bacteria has been undertaken for a taxonomic purpose. In this paper, the auxanographic method is described and discussed. Unlike other techniques in which the medium is supplemented by each substrate, at fixed concentration, the method described here consists in achieving diffusion of the 146 substrates tested as sole source of carbon and energy, in a medium without other energetic substrate.

The threonine-sensitive homoserine dehydrogenase and aspartokinase activities of Escherichia coli K12. Specific inactivation of the homoserine dehydrogenase activity by the affinity label, 2-amino-4-oxo-5-chloropentanoic acid.

2-Amino-4-oxo-5-chloropentanoic acid inactivates specifically the homoserine dehydrogenase activity of the bifunctional enzyme, aspartokinase I--homoserine dehydrogenase I. The aspartokinase activity remains essentially untouched and retains its threonine sensitivity. The inactivation of the dehydrogenase requires the covalent binding of one equivalent of the analogue per subunit.