Detection and determination of factor C--a regulatory protein--in Streptomyces strains by antiserum and monoclonal antibody.

Rabbit antisera and monoclonal antibodies were raised against factor C, a regulatory protein of Streptomyces griseus. ELISA and immunoblotting techniques suitable to determine and characterize factor C antigen was detected in all the 23 Streptomyces strains and variants examined thus far and in one Bacillus subtilis too. Depending on the strain analysed it has a molecular mass of 34,000 or 70,000 in mycelial homogenates.

Comparison of protein patterns of Streptomyces griseus spores from surface and submerged cultures.

Protein patterns of conidia produced by a Streptomyces griseus strain in submerged cultures and on solid media were compared. Cell-free extracts (30 000 X g supernatant) were prepared and analyzed on gradient SDS polyacrylamide gels. The protein patterns of both kinds of conidia were found to be practically identical, and they differed from protein patterns of the old vegetative hyphae in a characteristic way.

Protein patterns of Streptomyces griseus strains during development.

Protein patterns from mycelial extracts of Streptomyces griseus strains No. 45H and No. 52-1 were studied by one dimensional gradient PAGE with 20 cm run.

Streptomycin sensitivity of ribosomes isolated from a streptomycin-producing Streptomyces griseus.

The streptomycin sensitivity of ribosomes derived from a streptomycin-producing Streptomyces griseus was examined in a polyuridylic acid directed 14C-phenylalanine incorporating system. In order to get reproducible results it is essential to use cell-free extracts which do not inactivate streptomycin. This condition can be fulfilled by the combination of washed ribosomes of the streptomycin-producing strain and the 110 000 g supernatant of the streptomycin-nonproducing variant of S.

High molecular weight ribosomal ribonucleic acid from vegetative hyphae and spores of Streptomyces griseus.

High molecular weight ribosomal ribonucleic acids (rRNAs) were isolated from young vegetative cells and spores of a streptomycin non-producing Streptomyces griseus, and their electrophoretic mobility was compared to each other and to that of rRNAs of Escherichia coli K-12. The electrophoretic mobility of 23 and 16S rRNAs from vegetative cells and spores of S. griseus was identical, but the 23S rRNAs of streptomyces ribosomes migrated more slowly on polyacrylamide gel than those of E.

Proteolytic activity of subcellular fractions from Streptomyces griseus no. 45-H.

Subcellular fractions were prepared from Streptomyces griseus No. 45-H at different stages of life cycle, and their proteolytic activity was examined. The highest proteolytic activity was found in the 24- and 72- h-old vegetative hyphae, the lowest in the resting spores.

Ribosomal proteins from vegetative hyphae and from spores of Streptomyces griseus.

Ribosomes from vegetative cells and spores of Streptomyces griseus have been used to prepare ribosomal proteins for polyacrylamide gel electrophoresis. Differences in the gel electrophoretic profile of proteins from vegetative cells and spore ribosomes can be detected. Spore ribosomes were stable during the isolation process, but in the case of vegetative cell ribosomes it was necessary to use protease inhibitor to obtain reproducible results.