Proteomics Can Rise to the Challenge of Pseudogenes' Coding Nature.

Throughout the past decade, technological advances in genomics and transcriptomics have revealed pervasive translation throughout mammalian genomes. These putative proteins are usually excluded from proteomics analyses, as they are absent from common protein repositories. A sizable portion of these noncanonical proteins is translated from pseudogenes.

Comparative characteristics of DNA loop domains rearrangement in glioblastoma multiforme T98G and glioblastoma astrocytoma U373 cell lines under different culture conditions.

Background: The loop domain organization of chromatin, which plays an important role in transcription regulation, may depend on the cell functional state. The aim of this work was to investigate DNA loop reorganization upon functional transitions in two cell lines ‒ glioblastoma multiforme T98G and glioblastoma astrocytoma U373.

Materials And Methods: Single cell gel electrophoresis (the comet assay) was used to analyze the kinetics of the DNA loop migration from the nucleoids obtained from the lysed cells.

[Baculoviruses--a promising alternative to toxic chemicals in the battle against plant pests].

The economic, ecologic, medical, sanitary and hygienic expediency has been substantiated for the use of Baculoviridae family representatives in creation of preparations for the long-term integrated programs of pest control in natural biocenoses and agrobiocenoses. The program was based on the properties of this unique family. The problems and prospects of biotechnology of baculovirus preparation for pest control in plants in Ukraine have been discussed.

[Conceptual basis for defining viral insecticide harmlessness for human health and the environment].

The qualitative distinction of the new class of virus-based biopesticides from chemicals is the intracellular parasitism of the active agent in the organisms of the living biological host, its capacity to join natural microbiocenoses and to restrain the number of the destructive species over a long period of time. Thus, the program of the study of harmlessness of entomopathogenic viruses must be aimed at preventing the possibility of their adaptation to non-target animals, reproduction and the ability to provoke the non-specific infection. Other parameters such as toxicity and allergenicity of the preparations and their standardization will be investigated under the absence of such ability.