Publications by authors named "V V Ushakov"

Endogenous HS has been proposed to be a universal defense mechanism against different antibiotics. Here, we studied the role of HS transiently generated during ciprofloxacin (CF) treatment in M9 minimal medium with sulfate or produced by E. coli when fed with cystine.

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Article Synopsis
  • In Gram-negative bacteria, stress-induced growth arrest leads to the release of hydrogen sulfide, which comes from the breakdown of intracellular cysteine to prevent toxic accumulation.
  • Research using electrochemical sensors showed that both Gram-positive and Gram-negative bacteria produce sulfide during growth arrests caused by low nutrients or antibiotics, with notable changes in physiological parameters.
  • Differences in sulfide production between the two types of bacteria are attributed to the absence of glutathione in Gram-positive bacteria, suggesting that this process may represent a previously unknown natural source of hydrogen sulfide.
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Objective: To study the relationship of the parameters of immunity and systemic inflammation with the structural magnetic resonance imaging (MRI) parameters in patients with mild cognitive impairment (MCI) and pre-MCI undergoing neurocognitive rehabilitation to search for candidate markers of its effectiveness.

Material And Methods: The main group included 49 patients, aged ≥60 years, with MCI and pre-MCI with memory impairment, who underwent a course of neurorehabilitation for 5 weeks. The control group included 19 volunteers of similar age with a total MoCA score of ≥25, who did not have cognitive impairment and immuno-inflammatory disorders.

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Cysteine and its derivatives, including HS, can influence bacterial virulence and sensitivity to antibiotics. In minimal sulfate media, HS is generated under stress to prevent excess cysteine and, together with incorporation into glutathione and export into the medium, is a mechanism of cysteine homeostasis. Here, we studied the features of cysteine homeostasis in LB medium, where the main source of sulfur is cystine, whose import can create excess cysteine inside cells.

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