[Mapping of Rpn4p regions responsible for transcriptional activation of proteasome genes].

Rpn4p is positive and negative transcriptional regulator of the ubiquitin-proteasome system. At the same time, it is the extremely short-lived proteasome-associated protein and the proteasome substrate. Proteasome-dependent mechanisms of Rpn4p degradation is studied in details, but mechanisms of its action are not clear, and, first of all, functional domains is not defined.

[Isolation and identification of PACE-binding protein rpn4--a new transcription activator, participating in regulation of 26S proteosome and other genes].

A new upstream activating sequence (UAS) 5'-GGTGGCAAA-3' was detected in the promoters of 27 out of the 33 proteasomal genes and of several genes related to the ubiquitin-proteasomal system of Saccharomices cerevisiae. The sequence was termed proteasome-associated control element (PACE). Gel retardation assay revealed specific binding of PACE with an extracted protein.