[Formaldehyde metabolism in semicarbazide intoxication].

Subchronic administration of semicarbazide in the experiment with the rats was used to reduce the formaldehyde level in the organism in order to reveal the interaction between formaldehyde metabolism and biochemical parameters, which define the oxidant-antioxidant system condition and NO metabolism. It has been found that under semicarbazide impact the generation of free radicals, ROS, nitrite and nitrate were enhanced while aldehydes level was reduced that resulted from not only semicarbazide effect like the aldehydes acceptor, but the formaldehyde synthesis slowdown and acceleration of its transformation into format as well. We suppose that formaldehyde plays certain role in the development of connective tissue pathology.

[Role of formic acid in transformation of acetic and succinic acids in animal tissues].

Acetic and succinate acids KoA acyl derivatives interacting with formate were displayed to produce alpha-ketoacids--pyruvate and alpha-ketoglutarate. These acids also interact with formate and make pyruvic and malate acids, while alpha-ketoglutarate, evidently, tricarboxy acids. Interaction of formate with acetic and succinate acids inspite of occurring out of the tricarbone cycle increases the latter metabolic functions.

[Effect of catechins and proanthocyanidins on the initial stage of protein biosynthesis].

Catechins and proanthocyanidins have been studied for their effect on initial stage of the protein biosynthesis. It has been found that (+)-catechin in all concentrations studied does not influence the intensity of t-RNA aminoacylation by 14C-glycine and 14C-arginine. Proanthocyanidins, namely, dimers B-3, B-6 and trimer C2 in the concentration of 10(-3) M completely inhibit the initial stage of the protein biosynthesis.

[Change in the intensity of biosynthesis of proteins, lipids and glycogen in animal tissues during long-term administration of morphine].

The intensity of biosynthesis processes in animal organism has been studied as affected by long-term administration of morphine. It was established that morphine administration to rats for five weeks intensified protein biosynthesis in the brain, kidneys, skeletal muscles: specific radioactivity of blood serum proteins also increased. Incorporation of 2-/14C/glycine label to the brain, cardiac and skeletal muscles increased as affected by morphine: the label incorporation to the liver lipids decreased and that to the kidney and spleen lipids did not change.

[Features of metabolic disorders in the liver during morphine poisoning of various duration].

Administration of morphine into rats at a dose of 30 mg/kg within 6 days led to a decrease in total rate of tRNA aminoacylation in liver tissue. Content of lactate, pyruvate, malate and alpha-ketoglutarate was decreased within 6 days-long course of morphine administration, while content of lactate was only altered after 5 weeks of the intoxication. Adaptation reactions appear to be increased with time in long-term intoxication with morphine.