[Live xenogenic vaccine in the prevention of metastases of uveal melanoma].

By the results of experimental studies and by being guided by Protocol No. 22 dated January 22, 2004, by the Ministry of Health of the Russian Federation, the authors have conducted clinical trials of biotherapy used in oncological care. They describe the first experience in using xenovaccination in 35 patients with uveal melanoma to prevent hematogenic metastases.

Regulation of Anti-Tumor Activity Using Monoclonal Antibodies to Alpha-Fetoprotein Receptor and after Immunization with This Protein.

The object of this work was to study (i) the effect of monoclonal antibodies (mAb) to a receptor (R) of an oncofetal protein of an alpha-fetoprotein (AFP) on the survival rate and sensitivity of tumor target cells to the cytotoxic action of effector cells, (ii) the level of Ab to AFP-R in the blood serum of patients with malignant tumors (iii) the effect of blood serum with a high level of Ab to AFP-R on the survival rate of tumor cells in vitro, and also (iv) the effect of immunization of animals with an AFP-R preparation on subsequent development of a grafted tumor. It is shown that mAb to AFP-R of clones 2E1, 5C6 and 2B8 effectively bond to both mouse tumor cells and to human tumor cells. Monoclonal Ab to AFP-R of the studied clones do not affect the proliferation of tumor cells of mice and insignificantly inhibit the proliferation of human tumor cells.

[Monoclonal antibodies used to differentiate between poliovirus types 1 and 3].

A panel of mice hybrids was obtained, producing monoclonal antibodies (MCAB), which are capable to differentiate the vaccine and wild strains of polioviruses of the I and III types in the reaction of virus neutralization (RVN). The obtained MCAB activity was estimated on laboratory reference-strains and on the field isolates of polioviruses isolated in the territory of Ukraine in 1995-1998. The results of RVN with obtained MCAB were compared with the data of intratypical differentiation carried out with the use of standard MCAB from the Pasteur Institute as well as with results of the chain polymerization reaction (CPR) and immunoenzyme analysis (IEA) with cross-adsorbed sera.

[Isolation and characteristics of anti-adenovirus monoclonal antibodies in immunoenzyme and immunofluorescent reactions].

New monoclonal antibodies (MAbs) to adenovirus hexon, highly active in ELISA and immunofluorescent analysis, were prepared. According to competitive ELISA, new MAbs differed in their blocking activity and were directed to 2 different hexon epitopes. MAb 3H8 did not modify antigen binding of the rest MAbs labeled with peroxidase (PAb x Pox), and none of unlabeled MAbs suppressed the reaction of MAb x Pox 3H8.

Determination of Cross-Reactive (Common) Epitopes of Animal and Human Immunoglobulins Using Monoclonal Antibodies.

Monoclonal antibodies to cross-reactive epitopes of IgG and IgM of some animal species and man were produced. Specificity and cross-reactivity of the monoclonal antibodies were determined by ELISA, immunoblotting, immunodiffusion and immunoelectrophoresis. The presence of immunoglobulin epitopes common to some animal species and man that were localized on both native Ig molecules (conformational) and polypeptide chains (linear) was demonstrated.