Thermodynamic Description of Oxotransfer Processes Involving Molybdenum and Tungsten Enzyme Models: DFT Calculations and Calorimetry.

The enthalpy of the oxotransfer reaction of [BuN][WO(mnt)] (where mnt is maleonitriledithiolate) with PPh in an inert atmosphere in an acetonitrile solution was determined by calorimetry. The obtained enthalpy value (-93 ± 5) kJ mol differs from the enthalpy value of the reaction carried out by us earlier under aerobic conditions by (16 ± 9) kJ mol. The obtained results indicate the participation of atmospheric oxygen in the catalytic process.

CRISPR-Based Editing of the Gene.

gene is shown to have numerous diverse functions in plant development, including the regulation of embryo morphogenesis and maturation, hypocotyl elongation, flowering transition, etc. However, the functions of orthologs in different plant species have not been extensively studied. In this study, we obtained a line of , a model leguminous plant, carrying the loss-of-function mutation in the gene, orthologous to , using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated proteins (CRISPR/Cas9) genome editing system.

The Genes from the Intermediate Clade: Influence on the Somatic Embryogenesis in .

Transcription factors from the WOX family are well-known regulators of cell proliferation and differentiation in plants. Herein, we focused on several genes from the intermediate clade and checked their impact on somatic embryogenesis using the model legume object . As a result, we show that overexpression not only stimulates somatic embryogenesis in the embryogenic line, as it was shown previously, but can also induce somatic embryogenesis in the non-embryogenic line.

and Effects on the Embryogenic Callus Transcriptome in .

WOX family transcription factors are well-known regulators of plant development, controlling cell proliferation and differentiation in diverse organs and tissues. Several genes have been shown to participate in regeneration processes which take place in plant cell cultures in vitro, but the effects of most of them on tissue culture development have not been discovered yet. In this study, we evaluated the effects of gene overexpression on the embryogenic callus development and transcriptomic state in .

Chaperone Hsp70 (HSPA1) Is Involved in the Molecular Mechanisms of Sleep Cycle Integration.

The molecular mechanisms of sleep cycle integration at the beginning and the end of the inactive period are not clear. Sleep cycles with a predominance of deep slow-wave sleep (SWS) seem to be associated with accelerated protein synthesis in the brain. The inducible Hsp70 chaperone corrects protein conformational changes and has protective properties.