[Influence of prothymosin alpha and its mutants on activity of the p53 tumor suppressor].

Overexpression of the nuclear oncoprotein prothymosin a enhanced and, in a reciprocal experiment, down-regulation of endogenous prothymosin alpha by RNA interference approach inhibited transcriptional activity of the p53 tumor suppressor in the reporter gene assay. Ectopic expression of prothymosin alpha enhanced not only p53-dependent transcription, but also intracellular level of p53 in HeLa (but not HCT116) cells. Ability to stimulate p53-dependent transcription was lost by C-terminal mutants of prothymosin alpha with impaired nuclear accumulation, but not by N-terminal deletion mutants and by the double mutant of prothymosin alpha with impaired ability to bind Keap1, suggesting that prothymosinalpha-Keap1 interaction is dispensable for p53 response.

[Salvage thermochemotherapy in advanced refractory malignant tumors in children].

The data are presented on polychemotherapy given to 17 children with advanced refractory malignant tumors using whole body hyperthermia and hyperglycemia. All patients suffered tumor progression throughout treatment and afterwards. Adjuvant Roncoleukin (interleukin-2) was administered in 5.

[The use of a hydroxylapatitie-based biogenic composite material for eliminating intraosseous cavities].

A rational method to remove the intraosseous cavities by cystectomy, making use of biogenic composite material Colap, has been developed. This material stimulates reparative osteogenesis, creates the optimal conditions for bone wounds healing. A total of 46 patients were operated on for large extensive cysts of the jaws with filling of the postoperative bone defects with the biocomposite.