[Designing of hybrid human interferon alfa-2 strain-producers and the use of enteropeptidase for obtaining N-terminal methionine-free interferons].

A system for production of human interferon-alpha2a (IFN-alpha2a) and IFN-alpha2b lacking N-terminal methionine has been developed. Plasmids containing genes of hybrid IFN-alpha2 under the control of different promoters were constructed; a sequence encoding the enteropeptidase hydrolysis site being introduced in proximal part of the genes. As the result, 4 strains of Escherichia coli producing hybrid IFN-alpha2 have been obtained.

[Role of active forms of oxygen generated by phagocytes in the pathogenesis of diseases].

The review deals with the analysis of the properties of active forms of oxygen (AFO), generated by phagocytic cells, and their role in the development of a number of human diseases. Bacterial and viral infections contribute to the activation of the oxidizing metabolism of phagocytes. In the process of this metabolism the formation of toxic oxygen and nitrogen metabolites occurs.

[Use of column and thin layer chromatography for detection of vanillyl mandelic acid in urine].

The excretion of vanillylmandelic acid was measured by column chromatography of urinary samples on aluminum oxide with subsequent thin-layer chromatography on silica gel. Use of aluminum oxide allowed application of greater urine samples (up to 0.1% of 24-h diuresis) onto chromatographic plates and essentially improved the quality of separation of vanillylmandelic acid from other phenylcarbonic acids by thin-layer chromatography, as well as the specificity and reproducibility of measurements.

[The effect of gidazepam on the activity of the sympatho-adrenal system in operators working under the usual and complicated conditions].

In healthy volunteers a single dose (0.05 g) of hydazepam does not change, but in subjects with psychogenic stress-reactions one-week course of the drug (0.05 g/24 h) intensifies to optimum activation of the sympathoadrenal system in response to standard operator load, inducing prevalent excretion of norepinephrine but not of epinephrine in the urine and maintains a high level of dopamine and DOPA excretion.

[The effect of aflatoxin B1 on the human lymphocyte blast transformation reaction].

The effect of various aflatoxin B1 concentrations on human peripheral blood lymphocyte blast-transformation reaction was studied. There was aflatoxin B1-induced dose-dependent inhibition of DNA synthesis in the lymphocytes stimulated by phytohemagglutinin. The aflatoxin B1 concentrations causing 50% decreases in DNA synthesis were defined by the regression method.