[Determination of quantitative parameters of Escherichia coli phagocytosis by mouse peritoneal macrophages].

The fluorometric method was used to study guantitative parameters of phagocytosis of fluorescein-labeled Escherichia coli cells by mouse peritoneal macrophages. E. coli cells were conjugated with fluoresceinisothiocyanate (FITC) and then incubated with macrophages.

[Effect of short-term culture of blood lymphocytes in vitro on the ability of Fcgamma-receptors to bind IgG1 and IgG2 and mediation of antibody-dependent cytotoxicity].

The comparative study incubation influence at 37 degrees C on the lymphocyte Fc gamma-receptor (Fc gamma R) binding ability to IgG1 and IgG2 and on the antibody-dependent cell mediated cytotoxicity (ADCC) was performed. By means of the fluorometric binding assay it was determined that during 30 min of incubation the binding ability of Fc gamma R decreased while after 180 min of incubation it increased. Upon comparing both IgG1 and IgG2 binding abilities to lymphocytes no statistically important changes were noticed (p < 0.

[Use of a fluorescent method for detection of lymphocyte Fc-receptors].

FcR on chicken blood lymphocytes have been studied by fluorimetric binding assay. Binding data were analyzed by Scatchard's method. Temperature and time variations were investigated in order to optimise the binding conditions.

[Use of the method of lanthanide immunofluorescence analysis for detection of lymphocyte Fc-receptors].

Interaction of lymphocyte Fc-receptors (FcR) with the conjugate, containing immunoglobulin G (IgG) labeled with chelating compound of derivative diethylenetriaminepentaacetic acid (DTPA)-DTPA-Eu, was investigated. For optimal conditions in performing lanthanide immunofluorescence analysis (LIFA) with minimal quantity of lymphocyte, i. e.

[Biosynthesis of immunoglobulin G-binding factor in cattle lymphocytes].

Synthesis of the IgG-binding factor was estimated by the amount of labeled [14C]hydrolysate of proteins in the total synthesized de novo protein. The obtained data showed that the IgG binding factor which was synthesized by blood lymphocytes of the normal cattle and that suffered from chronic leukemia had a molecular weight of 72 kDa and was revealed before and after restoration of S-S bonds as one peptide.