[ON THE INFLUENCE OF PRENATAL HYPOXIA ON FORMATION OF THE OREXINERGIC SYSTEM AND SLEEP-WAKEFULNESS CYCLE IN EARLY POSTNATAL ONTOGENESIS OF RATS].

The role of orexin in the organization of sleep-wakefulness cycle (SWC) is well known. The aim of this study was to examine the terms of formation of the orexinergic system in the rat postnatal ontogenesis and to assess the role of orexin A in SWC organization under normal conditions and after prenatal hypoxia realized on days 14 and 19 of the embryogenesis. SWC was investigated in 30-day-old rats with elect- rodes implanted into the somatosensory and occipital cortex.

[Raction of dopaminergic and vasopressinergic systems in sleep deprivation in rats].

Six hours sleep deprivation experiments were carried out on rats after threefold injection of D1 dopamine receptor antagonist SCH 39 166. Immunohistochemical study of striatum revealed the increase in D1 and D2 dopamine receptor and glutamate immunoreactive material during the sleep deprivation and 2 h of postdeprivation period. The level of AMPA glutamate receptors increased under the sleep deprivation and decreased in the postdeprivation period.

[On filo- and ontogenetic development of dopaminergic regylation of wakefulness-sleep cycle in vertabrates].

The comparative immunohistochemical researches of dofamine containing neurons and fibers are carried uot in telencephalic and diencephalic departments of the brain in different vertebratts (adults rats, rats aged 14 and 30 days and frogs). For analysis of quantitative changes dynamics in thyrozinhydroxylase, D1 and D2 immunoreactive material in sleep-wakefulness cycle the model of sleepdeprivation is used. There are found the facts of morphofunctional correlations in the reactions of dophaminergic system during ontogeny and phylogeny.

The dopaminergic system of the telencephalo-diencephalic areas of the vertebrate brain in the organization of the sleep-waking cycle.

The aim of the present work was to study the involvement of the dopaminergic system of the telencephalic and diencephalic areas of the vertebrate brain in the organization of the sleep-waking cycle in cold-blooded and warm-blooded vertebrates. Immunohistochemical studies of tyrosine hydroxylase content, this being the key enzyme in dopamine synthesis, in the striatum, supraoptic and arcuate nuclei, and zona incerta of the hypothalamus of sturgeon and mammals (rats) of three age groups (14 and 30 days and adults), in conditions of tactile and sleep-deprivation stressors. In fish, transient stress was followed by the detection of tyrosine hydroxylase-immunoreactive cells in all parts of the brain.

[Dopaminergic system in the telencephalic and diencephalic brain regions in vertebrates in the sleep-wakefulness cycle organisation].

The aim of our study was to investigate the role of dopaminergic system in telencephalic and diencephalic brain regions of vertebrates in sleep-wakefulness cycle. The level of thyrosine-hydroxylase--the main enzyme in dopamine synthesis--was measured in striatum, zona inserta supraoptic and arcuate nuclea of hypothalamus in fish (Acipenceridae) and in mammals (rats) in ontogenesis (14-, 30-day old rats and adult animals) under tactile and sleep deprivation stresses. The thyrosine-hydroxylase-immunoreactive cells were revealed in all brain regions of fishes after a short-term stress.