Oscillatory Cs-137 Distribution Pattern in Scotch Pine Bark.

The vertical distribution of the anthropogenic radionuclide Cs-137 in the Scots pine (Pinus sylvestris L.) bark was studied in two model trees in the radioactive contamination zone of the Bryansk region. Each tree was divided into 10-cm bars from the trunk base to a length of 17 m, and the bark with the bast was separated from each bar to obtain a separate sample.

[Cloning of a new murine gene coding for a protein immunologically related to RecA protein from Escherichia coli].

Using the testis-specific murine cDNA library immunoscreening with the affine-purified polyclonal antibodies to the E. coli RecA protein, the 1730-bp fragment of the novel mouse gene was cloned. Northern hybridization of total RNA samples from mouse somatic and meiotic tissues showed that this gene was specifically expressed in mouse testis, producing a transcript of about 10 kb in size.

[Cloning and determination of the structure of a new human gene, homologous to Saccharomyces cerevisiae rad proteins].

Cloning of a novel Homo sapiens gene from cDNA libraries of human testes using immunoscreening procedure with affinity-purified polyclonal antibodies against E. coli RecA protein was carried out. The nucleotide sequence and chromosomal localization of this gene were determined.

Use of DNA fingerprinting for human population genetic studies.

DNA fingerprinting techniques have been used in population genetic studies on many different kinds of organisms. Here, we present new applications for multilocus DNA fingerprint probes in population studies and demonstrate the applicability of DNA fingerprinting to human population genetics, using M13 phage DNA as a probe. The new approach, which is based on a factor method of numerical coding of nonquantitative data (factor correspondence analysis-FCA), shows good agreement between population position, as indicated by the three principal factors, and ethnogenetic proximity.

Genetic differentiation in a captive population of the endangered Siberian crane (Grus leucogeranus Pall.).

DNA fingerprinting, followed by multivariate analysis of data, was used to characterize genetic heterogeneity in captive populations of the endangered Siberian and sandhill cranes. The genetic structure revealed reflected the natural population and species distributions. The relevant groups differed not only from each other, but also from interspecies and inter-population hybrids bred in captivity.