Publications by authors named "V Turilova"
Osteoporosis is a complex multifactorial bone disease with a strong genetic component. Among the various genes implicated in the progression of osteoporosis, those encoding G-protein-coupled receptors (GPCRs) play a crucial role in its pathogenesis. This superfamily of membrane receptors regulates myriad of cellular events including physiological and pathological processes in bone tissue.
View Article and Find Full Text PDFArticle Synopsis
- - The study focuses on correcting mutations in the CASR gene, which is crucial for regulating calcium levels in the body, specifically targeting mutations linked to a severe condition called neonatal primary hyperparathyroidism.
- - Researchers generated a CRISPR/Cas9 edited human induced pluripotent stem cell (hiPSC) line that successfully restored one of the mutations while preserving another, showing normal characteristics such as cell structure and growth potential.
- - The newly created hiPSC line will be used as a valuable resource for understanding calcium regulation disorders and exploring personalized medicine approaches to treat these conditions in the future.
Background: Chinese hamster ovary cell lines, also known as CHO cells, represent a large family of related, yet quite different, cell lines which are metabolic mutants derived from the original cell line, CHO-ori. Dihydrofolate reductase-deficient DXB-11 cell line, one of the first CHO derivatives, serves as the host cell line for the production of therapeutic proteins. It is generally assumed that DXB-11 is identical to DUKX or CHO-DUK cell lines, but, to our knowledge, DXB-11 karyotype has not been described yet.
View Article and Find Full Text PDFCytogenetic analysis of human myeloma cell lines L363, Karpas 707, RPMI 8226 and U-266 has been performed. Chromosome numbers retained near-diploid (L363, Karpas 707 and U-266) or increased to hypotriploid (RPMI 8226) during many years of maintenance of the cell lines in vitro. Based on G-banding analysis, the complex karyotypes with abnormalities of virtually all chromosome pairs were found in these cell lines but common chromosome translocations were not observed.
View Article and Find Full Text PDF