Cross contamination of intramyocellular lipid signals through loss of bulk magnetic susceptibility effect differences in human muscle using (1)H-MRSI at 4 T.

Cross contamination of intramyocellular lipid (IMCL) signals through loss of bulk magnetic susceptibility (BMS) differences was detected in human muscles using proton magnetic resonance spectroscopic imaging ((1)H-MRSI) at 4 T by varying nominal voxel sizes on healthy subjects. In soleus muscle the IMCL content estimated in 1.00-ml-sized voxels was 15% and 30% higher than that in 0.

Comparative microscopic study of the ovarian blood vessels.

The present study puts forth the morphometric analysis of modifications that occur in the ovarian structures (stroma, parenchyma, blood vessels) before and after menopause onset. Our study was performed on 30 human ovary specimens from patients with no history of genital pathology that were split in two groups (15 ovaries harvested from fertile women and 15 ovaries harvested from women at menopause). The specimens were processed following the classic histological technique of paraffin embedding and stained using Hematoxylin-Eosin technique and trichromic Goldner-Szeckelly technique.

Calculating glucose fluxes during meal tolerance test: a new computational approach.

A new calculation method is proposed to quantify the endogenous glucose production (EGP), the glucose appearance rate due to meal ingestion (R(a meal)), and the glucose disposal (R(d)) during a three-tracer study design. The method utilizes the maximum likelihood theory combined with a regularization method to achieve a theoretically coherent computational framework. The method uses the two-compartment formulation of the glucose kinetics.

LC/MS quantitative study of glucose by iodine attachment.

We explored the potential of iodine attachment to improve the sensitivity of glucose measurement by LC/MS. After sample preparation, glucose was separated by normal phase chromatography, followed by anionization by I(-)-attachment prior to MS by post-column addition of a methanolic solution of iodoform. Iodine is capable of forming an anionic adduct with neutral monosaccharides in negative ion mode electrospray mass spectrometry.

Cerebellar atrophy--a comparative microscopic study.

The cerebellum is required for the coordination of fine movement. In health, it provides corrections during motion which are the basis for precision and accuracy, and it is critically involved in motor learning and reflex modification. Disease of the cerebellum or its connections leads to incoordination.