Physicochemical cell disruption of sp. for recovery of polyhydroxyalkanoates: future bioplastic for sustainability.

Polyhydroxybutyrate (PHB) is known for wide applications, biocompatibility, and degradability; however, it cannot be commercialized due to conventional recovery using solvents. The present study employed mechanical cell-disruption methods, such as Pestle and mortar, sonication, and glass bead vortexing, for solvent-free extraction of PHA from sp. Different time intervals were set for grinding (5, 10, 15 min), sonicating (1, 3 and 5 min), and vortexing (2, 5 and 8 g glass beads with 5, 10 and 15 min each) hence studying their effect on cell lysis to release PHA.

Sequestration of simulated carbon dioxide (CO) using churning cementations waste and fly-ash in a thermo-stable batch reactor (TSBR).

The degrees of mineral carbonation in (a) construction and demolition waste (C&DW) and (b) a mixture of cement and fly ash were studied through a dynamic experimental method to determine the variation in the rate and extent of CO sequestration achievable under simulated outdoor conditions. A number of experiments were performed in a self-designed rotating batch reactor by churning the two samples together with CO, which was passed through the mixtures by using water vapor as the medium of transfer. At an injection flow rate of 1 L/min for CO, the theoretical extent of carbonation was observed to be 39.