Publications by authors named "V T Potapov"

Article Synopsis
  • Direct methods for assessing DNA polymerase fidelity are straightforward, but measuring RNA polymerases and reverse transcriptases is trickier due to extra preparation steps that can lead to errors.
  • The new method, Roll-Seq, uses single molecule real-time sequencing to evaluate the fidelity of RNA polymerases and reverse transcriptases simultaneously, by generating long concatemeric cDNA from a circular RNA template.
  • Roll-Seq results showed that while some reverse transcriptases had consistent substitution rates, others demonstrated lower fidelity during second-strand synthesis, highlighting the importance of RNA structure on polymerase accuracy.
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We studied the possibility of using 4-hexylresorcinol to increase the efficiency of anti-mycobacterial chemotherapy. In an in vitro experiment, 4-hexylresorcinol increased the efficiency of rifampicin, kanamycin, and isoniazid against Mycobacterium smegmatis by 3-5 times. Experiments in sanitation of BALB/c mice infected with M.

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Commercially synthesized genes are typically made using variations of homology-based cloning techniques, including polymerase cycling assembly from chemically synthesized microarray-derived oligonucleotides. Here, we apply Data-optimized Assembly Design (DAD) to the synthesis of hundreds of codon-optimized genes in both constitutive and inducible vectors using Golden Gate Assembly. Starting from oligonucleotide pools, we synthesize genes in three simple steps: (1) amplification of parts belonging to individual assemblies in parallel from a single pool; (2) Golden Gate Assembly of parts for each construct; and (3) transformation.

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Highly efficient and convenient methods for the preparation of 35 novel derivatives of 9-selenabicyclo[3.3.1]nonane and 9-selenabicyclo[3.

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In the paper, the effect of spontaneous Brillouin scattering (SpBS) is analyzed as a noise source in distributed acoustic sensors (DAS). The intensity of the SpBS wave fluctuates over time, and these fluctuations increase the noise power in DAS. Based on experimental data, the probability density function (PDF) of the spectrally selected SpBS Stokes wave intensity is negative exponential, which corresponds to the known theoretical conception.

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