Biological and chemical detections in adsorbent layer for monitoring microbial production of primycin.

Primycin is a macrolide antibiotic complex produced in microbiological fermentation processes. The microbial production of primycin requires an in-process analytical method suitable for monitoring the level of the active agents. In this paper, a method fulfilling the described requirement is presented.

Selective quantitative determination of tobramycin from fermentation broth.

A derivative of Rhizobium meliloti 41 was constructed (strain GY654) which was resistant to apramycin and kanamycin but remained sensitive to tobramycin. Strain GY654 selectively measures tobramycin and 6"-O-carbamoyltobramycin in the presence of apramycin, kanamycin and 6"-O-carbamoylkanamycin B, therefore it is suitable for the rapid quantitation of 6"-O-carbamoyltobramycin and tobramycin in fermentation broths of Streptomyces tenebrarius and solvents containing antibiotic mixtures.

Quantitative measurements of human bone enzymes.

Up to now quantitative analysis of human bone enzymes is a part of osteology which is not evaluated sufficiently. The lack of standardized methods, fresh bone and reliable methods of bone disintegration seem to impede these experiments mainly. The authors have elaborated a complex method for quantitative measurements of human bone enzymes and they describe the practical use of this method.